'Parental plasmid' presentation slideshows

Extension of the oligonucleotide primers generates a mutated plasmid containing nicks and

Take any plasmid in which the gene of interest is inserted. Multiply this plasmid within a methylating bacteria. ( While plasmid DNA isolated from almost all of the commonly usedE. coli strains (dam+) is methylated and is a suitable template formutagenesis, plasmid DNA isolated from the

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