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Pharmacognostic validation of root of Hemidesmus indicus (Linn.) R.Br.

Pharmacognostic validation of root of Hemidesmus indicus (Linn.) R.Br.

Hemidesmus indicus (Linn.) R.Br. Family: Asclepiadaceae, is commonly known as ‘Indian sarasaparilla’. A perennial climber and growing widely in upper Gangetic plains and eastwards of Bengal and from central to southIndia. It is traditionally used for curing various ailments like stomach pain, fever, veneral disease, rheumatism and also act as a blood purifier. This traditionally useful part was standardized based on the macroscopic, microscopic, physico-chemical and chromatographic. And also estimated for its microbial limits ,pesticides, Aflatoxin and heavy metals. Preliminary phytochemical screening confirms the presence of sapnonins, tannins, resins, essential oils, sterols and glycosides determination of total saponin content.

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481 views • 10 slides
Antidiabetic, antihyperlipidaemic and hepatoprotective activity of methanolic extract of Ruellia tuberosa Linn leaves in

Antidiabetic, antihyperlipidaemic and hepatoprotective activity of methanolic extract of Ruellia tuberosa Linn leaves in

Ruellia tuberosa Linn belongs to family acanthaceae is a large sized plant distributed throughout India, Srilanka and Nepal. The aim of the present study was to evaluate the anti diabetic potential of methanolic extract of Ruellia tuberosa linn leaves in normal and alloxan induced diabetic rats. The Preliminary phytochemical screening shows the presence of carbohydrates, glycosides, phytosteroids, flavonoids, tannins, fixed oils & fats. Diabetes was induced in Albino rats by administration of alloxan monohydrate (150mg/kg, i.p).The methanol extract of Ruellia tuberosa linn leaves at a dose of 100 and 200mg/kg of body weight was administered at single dose per day to diabetes induced rats for a period of 14 days. The effect of methanol extract of Ruellia tuberosa linn leaves on blood glucose, serum lipid profile [total cholesterol (TC), triglycerides (TG), high density lipoprotein – cholesterol (HDL-C), low density lipoprotein – cholesterol (LDL-C), very low density lipoprotein – cholesterol (VLDL-C) and serum protein, albumin, globulin, serum enzymes [serum glutamate pyruvate transaminases (SGPT), serum glutamate oxaloacetate transaminases (SGOT), alkaline phosphatase (ALP)], were measured in the diabetic rats. The methanol extract of Ruellia tuberosa linn leaves elicited significant reductions of blood glucose (P<0.05), lipid parameters except HDL-C, serum enzymes and significantly increased HDL-C at the dose of 200mg/kg was compared with the standard drug Glibenclamide (5gm/kg). From the above results, it is concluded that methanol extract of Ruellia tuberosa linn leaves possesses significant antidiabetic, antihyperlipidaemic and hepatoprotective effects in alloxan induced diabetic rats.

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Secondary metabolites investigation and its derivatives on Cassia occidentalis

Secondary metabolites investigation and its derivatives on Cassia occidentalis

The comparative studies of secondary metabolites investigation used in pharmacognostic drug research using qualitative test is been performed on Cassia occidentalis leaf as well as stem ; belongs to family Ceasalpinieaceae.This plant is of high economic value due to the presence of antioxidant and antimicrobial activity. The samples extracted using various solvents like Ethanol, Methanol, Ethyl acetate and water to detect the presence of active components. The phytochemical screening revealed the presence of carbohydrate glycosides, alkaloids, phenols, flavanoid, aminoacid, coumarine and phytosterols. Since it contains high proportion of phenols and alkaloid, it is reliable to possess antioxidant and anticancer activity.

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Antibacterial Activity and Phytochemical Analysis of Euphorbia hirta Against Clinical Pathogens

Antibacterial Activity and Phytochemical Analysis of Euphorbia hirta Against Clinical Pathogens

Euphorbia hirta powdered plant material was extracted using two solvents distilled water and methanol. The water extract provide higher yield and also more antibacterial effectiveness than methanol extract used. The well diffusion method was used to determine the antibacterial activity against Escherchia coli, Salmonella typhi, Bacillus subtilis, pseudomonas aeruginosa, Klebsiella pneumoniae. E.hirta plant was investigated for some of their components and antimicrobial activity of their leave extract against bacteria. Phytochemical screening of the crude extracts revealed the presence of tannins, saponins, steroids, flavonoids, terpenoids, and alkaloids.In GC MS analysis, four bioactive phytochemical compounds were identified in the methanolic extract of Euphorbia hirta, the components were identified by comparing their relation indices and mass spectra Fragmentation patterns with those stored on the MS Computer library. This presence of these bioactive constituents has been linked to the antimicrobial activity of the plant material. Dry leaf extracts of E. hirta produced the highest zones of inhibition on Salmonella typhi. The herb E. hirta can be used as source of oral drugs to fight infections caused by susceptible bacteria. Shanmugam A | Subha S | Logeshwaran S "Antibacterial Activity and Phytochemical Analysis of Euphorbia hirta Against Clinical Pathogens" Published in International Journal of Trend in Scientific Research and Development (ijtsrd), ISSN: 2456-6470, Volume-2 | Issue-1 , December 2017, URL: https://www.ijtsrd.com/papers/ijtsrd5939.pdf Paper URL: http://www.ijtsrd.com/biological-science/biotechnology/5939/antibacterial-activity-and-phytochemical-analysis-of--euphorbia-hirta-against-clinical-pathogens/shanmugam-a

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Extraction and isolation of gallic acid from self-generated fermentation system of Terminalia chebula

Extraction and isolation of gallic acid from self-generated fermentation system of Terminalia chebula

Myrobalan (Terminalia chebula) is a potential raw material for the production of gallic acid. It is a rich source of tannins and contains up to 40% of total tannins as gallotannins. The present study involves the fermentation of Myrobalan used as a substrate for fermentation using arishta containing tannase producing microorganism resulting in the biotransformation of Myrobalan tannins to gallic acid which is extracted, isolated and characterized. The self-generated fermentation system, arishta was prepared by forming a decoction of Myrobalan which was allowed to ferment for one month. Then after the fermentation was complete the gallic acid was extracted and isolated by partitioning it with ethyl acetate. The gallic acid crystals obtained upon recrystallization was characterized by phytochemical screening, TLC, IR, NMR and XRD. The completion of fermentation process was confirmed when the pH of the formulation was found to be 4.28. The extract was screened for the presence of tannins, which was confirmed by the appearance of bluish black precipitate upon reaction with Ferric chloride solution. Further the structural characterization of gallic acid was confirmed by TLC, FTIR, NMR and XRD.

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138 views • 5 slides

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