'Real time pcr method' presentation slideshows

Setting of the threshold in the middle of the linear part in a logarithmic view

130 000 000. 13 000 000. 4200. Y(X 0 )=Y 0. 1 300 000. 130 000. 17 000. Amplification : +. 420. 42. Intermediate precision. No amplification : -. for 2, 4, 8, 10 and 16 runs data are grouped. ~ 10 calibrations (run) needed to set a robust platform cut-off value. bias. variance.

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