'Rp hplc' presentation slideshows

520 views • 25 slides

451 views • 25 slides

Baculovirus transfer vector pAcGP67B

PvRII Expression. Plasmid for Expression in Insect Cells. PvRII. pAcR2H. Baculovirus transfer vector pAcGP67B. {From Becton Dickenson, PharMingen, Baculovirus Expression Manual, 2001}. +. Viral DNA. pAcR2H. Sf cell. a) Reinfect for Protein prod. b) Amplify for Virus titre.

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A Novel RP-HPLC Method for the Simultaneous Assessment of Olmesartan, Amlodipine and Hydrochlorothiazide and its Applica

The development and validation of a simple, rapid, precise and accurate RP-HPLC has been described for the simultaneous assessment and invitro dissolution of Olmesartan (OLM), Amlodipine (AML) and Hydrochlorothiazide (HCTZ) in their combined dosage forms. Separation was performed on Inertsil ODS-3 column (C18, 150 mm x 4.6 mm, 5 u03bcm) using photodiode array (PDA) detection at 255 nm. The mobile phase consisted of phosphate buffer (pH 3.5) and acetonitrile, in a gradient program (time in minutes/acetonitrile %: 0.00/30, 1.00/30, 8.30/70 and 9.00/30) at a 1.3 ml min-1 flow rate. Analytes were perfectly resolved with retention times of 2.59, 4.99 and 7.48 min for OLM, AML, and HCTZ, respectively. Following the recommended procedure, linear calibration graphs extended for 0.20-28.00, 0.05-7.00 and 0.12-17.50 u00b5g ml -1 with detection limits of 0.021, 0.002 and 0.012 u00b5g m; -1 and quantitation limits of 0.063, 0.036 and 0.007 u00b5g ml -1 for the assay and in-vitro drug release of OLM, AML and HCTZ, respectively. In-Vitro dissolution revealed that >96% of the labeled OLM, AML and HCTZ were released from their combined tablets within 20 min. The developed method was validated following the ICH guidelines regarding the system suitability, specificity, linearity, accuracy, precision, and robustness.

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A rapid RP-HPLC method for the estimation of famciclovir in tablet dosage forms

A rapid and simple RP-HPLC chromatographic method has been developed for the estimation of famciclovir in API and tablet dosage form.

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Analytical Method Development and Validation of Teneligliptin by using RP HPLC with ICH Guidelines

Teneligliptin is drug used against type 2 diabetes mellitus and it is also a member of class of anti diabetic drugs known as dipeptidyl peptidase 4 inhibitors or gliptins . A simple, sensitive and accurate RP HPLC method has been developed for the determination of Teneligliptin in bulk formulation. The max of the Teneligliptin was found to be 246 nm in Methanol Phosphate buffer pH 3 70 30 v v . The method shows high sensitivity with linearity 10 to 50 u00b5-g ml regression equation y = 54647x 74133 r2 = 0.9968 . The various parameters according to ICH guidelines are followed for validating and testing of this method. The Detection limit and quantitation limit were found to be 0.109 u00b5-g ml 1 and 0.3305 u00b5-g ml1 in Methanol Phosphate buffer pH 3 70 30 v v respectively. The purity of tablet formulation was found to be 99.57 . The results demonstrated that the procedure is accurate, specific and reproducible RSD 2 , and also being simple, cheap and less time consuming and appropriate for the determination of Teneligliptin in bulk and pharmaceutical formulation. Dr. Pradnya Lokhande "Analytical Method Development and Validation of Teneligliptin by using RP-HPLC with ICH Guidelines" Published in International Journal of Trend in Scientific Research and Development (ijtsrd), ISSN: 2456-6470, Volume-3 | Issue-3 , April 2019, URL: https://www.ijtsrd.com/papers/ijtsrd21735.pdf Paper URL: https://www.ijtsrd.com/chemistry/chromatography/21735/analytical-method-development-and-validation-of-teneligliptin-by-using-rp-hplc-with-ich-guidelines/dr-pradnya-lokhande

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RP-HPLC method for estimation of itopride hydrochloride from tablets dosage form

An isocratic reversed phase high-performance liquid chromatographic method with ultraviolet detection at 220 nm has been developed for the quantification of itopride hydrochloride in tablet dosage form. The quantification was carried out using C 8 column (250 mm × 4.6 mm), 5-µm particle size SS column. The mobile phase comprised of two solvents (Solvent A: buffer 1.4 mL ortho-phosphoric acid adjusted to pH 3.0 with triethyl amine and Solvent B: acetonitrile). The ratio of Solvent A: Solvent B was 75:25 v/v. The flow rate was 1.0 mL -1 with UV detection at 220 nm. The method has been validated and proved to be robust. The calibration curve was linear in the concentration range of 80-120% with coefficient of correlation 0.9995. The percentage recovery for itopride HCl was 100.01%. The proposed method was validated for its selectivity, linearity, accuracy, and precision. The method was found to be suitable for the quality control of itopride HCl in tablet dosage formulation.

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Analytical Methods Development and Validation of Naproxen and Sumatriptan by RP HPLC

Aim and objective A simple, pru00e9:cised, accurate method was developed for the estimation of Crizotinib by RP HPLC technique. Chromatographic conditions used are stationary phase BDS 250x4.6 mm, 5u00b5-. Mobile phase buffer Methodology Acetonitrile in the ratio of 60 40 and flow rate was maintained at1ml min, detection wave length was 267 nm, column temperature was set to 30o C and diluents was methanol water System suitability parameters were studied by injecting the standard five times and results were well 50 50 , Results and Discussion Conditions were finalized as optimized method under acceptance criteria. Linearity study was carried out between 25 to 150 levels, r2 value was found to be as 0.999. Precision was found to be 1.26 for repeatability and 0.93 for intermediate precision. LOD and LOQ are 0.080 ug ml. By using above method assay of marketed formulation was carried out 100.24 was present. Prapulla Putta "Analytical Methods Development and Validation of Naproxen and Sumatriptan by RP HPLC" Published in International Journal of Trend in Scientific Research and Development (ijtsrd), ISSN: 2456-6470, Volume-3 | Issue-4 , June 2019, URL: https://www.ijtsrd.com/papers/ijtsrd23582.pdf Paper URL: https://www.ijtsrd.com/pharmacy/analytical-chemistry/23582/analytical-methods-development-and-validation-of-naproxen-and-sumatriptan-by-rp-hplc/prapulla-putta

81 views • 3 slides