'Size exclusion' presentation slideshows

MISS. RAHIMAH BINTI OTHMAN (Email: rahimah@unimap.my)

Chapter 6: chromatography. MISS. RAHIMAH BINTI OTHMAN (Email: rahimah@unimap.edu.my). COURSE OUTCOMES. OUTLINES. Basic principles and applications of chromatography process. Types of separation in chromatography. Basic design of chromatography.

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Mechanisms of Enzyme Action

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How to Start a Biotechnology Program

How to Start a Biotechnology Program. Mary Jane Kurtz, Ph.D NBC 2 Consultant mjkurtz@biomanufacturing.org. Why Biotechnology? A number of good reasons. Academics Technical skills Biotechnology as a future career Biotechnology is exciting Biology can be seen with a molecular perspective

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Protein Purification: From industrial enzymes to cancer therapy

Protein Purification: From industrial enzymes to cancer therapy. 2. Jim DeKloe Solano Community College james.dekloe@solano.edu Bio-Rad Curriculum and Training Specialists : Sherri Andrews, Ph.D. (Eastern US) sherri_andrews@bio-rad.com Leigh Brown, M.A. (Central US)

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Chapter 28 Liquid Chromatography

Chapter 28 Liquid Chromatography. Small particles (3-10 ? m) give high performance but require high pressure 1. Scope of LC Four types of liquid chromatography - Partition Adsorption (liquid-solid) Ion-exchange Size exclusion or gel. 1 Scope of LC.

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Chromatography

Chromatography. The use of chromatography in the organic lab Christian Tortzen. Agenda. Introduction Very little theory Stationary phase Mobile phase Analytical methods Preparative methods Gravitational/Flash chromatography Dry Column Vacuum Chromatography. Theory.

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Protein-protein interactions

Protein-protein interactions. Masoud Youssefi, MD,PhD Division of microbiology/virology. Introduction. important field in cell biology, biochemistry Localization and trafficking posttranslational modifications signaling networks also important field in viral replication

633 views • 23 slides

Microencapsulation

Microencapsulation. Eric Lee, Yik Ning Wong, Miguel Benson, John Harrison, Albert Kwansa Client: Dr. Craig Atwood Advisor: Professor William Murphy. H ypothalamus. -. -. Target Cells. GnRH. LH & FSH. Testosterone. +. Anterior P ituitary. -. -. FSH. LH. Testosterone. Inhibin. +.

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Lecture 12 HPLC

Lecture 12 HPLC. Lecture 12 HPLC. GC setup. LC setup. LC Injector. Back to Van Deempter. A +. ?. H . C ?. Back to Van Deempter. d p 2 ?. +. C e d p. ?. H . ?. D MP. Mobile Phase. d p 2 ?. +. C e d p. ?. H . ?. D MP. LC Columns Particle Size (d p ). d p 2 ?. +.

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Lecture 6 Working with proteins

Lecture 6 Working with proteins. Protein purification. Source of protein Overexpression of protein Fractionation procedures Solubility Salting out ((NH 4 ) 2 SO 4 ) pH changes Size Centrifugation Size exclusion (gel filtration) Charge/polarity Ion exchange chromatography

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40-

1 2 3 4 5 . kDa. 85-. 70-. 60-. 50-. 40-. 30-. 25-. 20-. 15-. Additional file 1: Figure S1. SDS-PAGE analysis of overproduced and purified B. cereus Fnr .

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