'Specific binding' presentation slideshows

Transcription

Transcription. RNA Polymerases and General Transcription Factors. Subjects, covered in this lecture. A short overview Structure and mechanism of RNA polymerases Eukaryotic promoters General transcription factors Elongation factors

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Immunophenotyping and applications of cell analysis in the hematology laboratory

Immunophenotyping and applications of cell analysis in the hematology laboratory. J.Paul Robinson Professor of Immunopharmacology & Biomedical Engineering April 5, 2005. What can flow cytometry be used for?. Clinical and Research. Immunology Hematology Pathology Microbiology Genetics.

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Chemical syntheses of nu c leob ases , nu c leosid es , nu c leotid es a oligonu c leotid es

Chemical syntheses of nu c leob ases , nu c leosid es , nu c leotid es a oligonu c leotid es. Synthesis of pyrimidine bases. Transforma tions of pyrimidine bases. Syntheses of puri ne bases. Transforma tions of puri ne bases. Synthesis of nu c leosid es. Synthesis of nu c leosid es.

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MCB- Signal Transduction Lecture 1

MCB- Signal Transduction Lecture 1. General Concepts of Signal Transduction Cell Communication Types of Receptors Molecular Signaling Receptor Binding Scatchard Analysis Competitive Binding Second Messengers. Signaling throughout Evolution. Bacteria Sense nutrients

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Molecular interactions observed in a real time

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Lecture 21 Vesicular Traffic

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Functional Neuroimaging Lab School of Psychology University of Newcastle

Comparison of methodologies for the assessment of dopamine receptor binding in subregions of the striatum. Sharna Jamadar Mentor : Julie Price PET Modality Coordinator: Jonathan Carney. Functional Neuroimaging Lab School of Psychology University of Newcastle

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Immunolabeling of Cells and Transmission Electron Microscopy

Immunolabeling of Cells and Transmission Electron Microscopy. Ike Miguel June 30, 2006 ABE Workshop 2006. Purpose. Using TEM to label specific sites in Arabidopsis thaliana. Goal Overview of Immunolabeling Preparation of samples and viewing Observations of results Thoughts.

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Ch 9:T cell receptor

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Chromatography

Chromatography. Desalting and Affinity. Chromatography. Technique to separate components of a mixture by passing them through a matrix. A solvent is used for carrying sample mixture through the matrix Separation occurs because each compound in a mixture interacts differently with the matrix.

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Jui-Ming (Ryan) Yang

Label-Free Protein Biosensor Based on Aptamer-Modified Carbon Nanotube Field-Effect Transistors Kenzo Maehashi, Taiji Katsura, Kagan Kerman, Yuzuru Takamura, Kazuhiko Matsumoto, and Eiichi Tamiya Anal. Chem. 2007, 79, 782-787. Jui-Ming (Ryan) Yang. Outline. Background CNT applications

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plasma

K 1. k 7. K 5. k 3. k 2. k 8. k 6. k 4. Full 6-compartments. injection. t=0. V. IV. plasma. II. III. K 9. urine. K 1. K 2. Extracellular Fluid (ECF). injection. t=0. plasma. ECF. K 9. urine. K 1. K 5. k 2. k 6. Slow Space. injection. t=0. plasma. ECF. slow. K 9.

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Magnetic particles conjugation kits

With high efficacy and non-specific binding ability, our fast and easy magnetic particles conjugation kits exhibit promising properties as useful tools for antibodies and other biomolecules conjugation processes. https://www.cd-bioparticles.com/product/magnetic-particles-conjugation-kits-list-21.html

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Kd or IC50: concentration that gives 50% maximal binding; measure

TYPICAL BINDING CURVE. Maximum Number of receptors. SPECIFIC BINDING (NUMBER OF RECEPTORS OCCUPIED). Kd. Concentration of Drug Used. Kd or IC50: concentration that gives 50% maximal binding; measure of AFFINITY of the drug for the receptor. efficacy. ED50.

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1 brood

total CORT. 60. 50. 40. Total CORT (ng/ml). 30. 20. 1 brood. 10. 0. 0-3 minutes. 30 minutes. 600. CBG capacity. 500. 400. Specific Binding (nM). 300. 200. 100. 2-3 broods. 0. pugetensis. oriantha. gambelii. 3.5. Free CORT. 3. 2.5. 2. Free CORT (ng/ml). 1.5. 1.

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O Duss et al. Nature 000 , 1-5 (2014) doi:10.1038/nature13271

Sequential and specific binding of the RsmE protein to the RsmZ sRNA. O Duss et al. Nature 000 , 1-5 (2014) doi:10.1038/nature13271.

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magnetic beads kit

http://www.cd-bioparticles.com/l/Magnetic-Particles-Conjugation-Kits_20_307_0.html

81 views • 1 slides