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2.6.2 and 2.6.4

2.6.2 and 2.6.4. 2.6.2: USFWS Capacity for eDNA Sampling for Early Detection 2.6.4: USFWS Capacity for eDNA Sample Processing. Mike Weimer. Introduction and Objectives.

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2.6.2 and 2.6.4

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  1. 2.6.2 and 2.6.4 2.6.2: USFWS Capacity for eDNA Sampling for Early Detection 2.6.4: USFWS Capacity for eDNA Sample Processing Mike Weimer

  2. Introduction and Objectives 2.6.2: Will provide USFWS Fish and Wildlife Conservation Office facilities the initial resources to conduct integrated long‐term early detection activities with a particular focus on locations in southern Lake Michigan, western Lake Erie and other invasional hotspots. 2.6.4: Will provide USFWS La Crosse Fish Health Center with initial resources to develop adequate staffing, equipment, and laboratory space to begin processing eDNA samples.

  3. Current Knowledge • FY 2011 Action: • Task 2.6.2: • Testing and refinement of capabilities for monitoring and sampling eDNA outside the CAWS • Development of an eDNA sampling protocol that will be incorporated into long term monitoring strategy in collaboration with participating agencies.

  4. Current Knowledge (continued) • FY 2011 Action: • Task 2.6.4: • Development of the capacity for the systematic analysis of eDNA samples - initially from the CAWS and then other locations around the Great Lakes • Assist collaborating agencies in validation of eDNA calibration and sample processing as facility comes on line decreasing turn‐around time for results • Asian carp highest priority for sample processing followed by other high risk AIS

  5. Future Plans 2.6.2 • Fully develop the capacity for implementing an eDNA sampling program at USFWS Great Lakes Fish and Wildlife Conservation Offices. Q4 - 2012 • Support the establishment of a biologically based statistically tenable eDNA sampling protocol for use basin wide. Q4 - 2011 • Implement an eDNA draft sampling protocol at other areas of concern with focus on southern Lake Michigan, western Lake Erie and other potential hotspots. Q4 - 2012

  6. Future Plans 2.6.4 • Meet with USACOE ERDC personnel to establish timeline and feasibility of eDNA sampling technology transfer. Q2 - 2011 and ongoing • Develop guidelines and process for eDNA technology transfer. Q2/Q3 - 2011 and ongoing • Establish new lease agreement with GSA allowing for remodel and expansion of the La Crosse FHC facility. Q3 - 2011.

  7. Future Plans 2.6.4 (continued) • Remodel and expand La Crosse FHC facility, purchase needed equipment and supplies. Q3 - 2012 • Hire appropriate personnel. Q4 - 2011.

  8. Technological or Other Hurdles • 2.6.2: • Weather and access to sampling sites • Quality Assurance/Quality Control (QAQC) process for sample contamination • Calibration of eDNA technology is needed • 2.6.4: • Calibration results/lack of results may influence timeline for transfer to USFWS • Weather may influence construction timelines

  9. Data Gaps • Calibration work will be key to quantify what an eDNA detection specifically means • Marker development may not cover all target species • La Crosse initial sample processing capacity will be 50 samples per week which is less than is currently being processed

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