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Membrane arrays used to analyze protein content of APOSEC for cytokines and angiogenic factors. Detection conducted in cell culture supernatants from apoptotic PBMC. Experiments carried out following manufacturer’s protocol.
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(a) Cytokine Array 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 A B C D E
(b) Angiogenesis Array 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 A B C D E F Supplementary Figure 1: Membrane array analysis of APOSECH. Membrane arrays were utilized to scan the protein content of APOSECH for various (a) cytokines and (b) angiogenic factors. Supplementary Figure 1 Methods Membrane arrays for the detection of cytokines and angiogenic factors in cell culture supernatants derived from apoptotic PBMC were performed to scan the protein content of APOSEC (Proteome Profiler Array Ary005 and Ary007, R&D Systems, USA). Experiments were performed according to the manufacturer’s protocol.