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Genetic Engineering/Recombinant DNA

Genetic Engineering/Recombinant DNA. The direct manipulation of an organisms genome using biotechnology New DNA is inserted into a host genome Host organism transcribes and translates that DNA into a protein not normally manufactured by that organism

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Genetic Engineering/Recombinant DNA

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  1. Genetic Engineering/Recombinant DNA The direct manipulation of an organisms genome using biotechnology New DNA is inserted into a host genome Host organism transcribes and translates that DNA into a protein not normally manufactured by that organism Important/Interesting Examples: Insulin(and other biopharmaceutical)producing bacteria & animals, GloFish, GMO’s

  2. Gene Therapy • The use of DNA as a drug to treat disease by delivering therapeutic DNA into a patients cells. • Good gene is inserted using a virus to replace a mutated gene. • Has been used to treat: Choroderemia, Immunodeficiency diseases, chronic lymphocytic leukemia, Beta-thalassemia, and color-blindness in dogs • Speculative Uses: • Designer children and curing genetic diseases in vitro • Gene Doping to improve athletic performance

  3. GENE THERAPY

  4. DNA Fingerprinting The isolation, imaging and matching of DNA sequences Used to identify individuals by matching their DNA profiles Parental Testing, Criminal Investigations, Identification of suspects & victims, natural disasters, acts of terrorism. Restriction enzymes are used to cut DNA into small pieces that are then separated on a GEL.

  5. BioInformatics • Using computers to store, organize, and compare the genomes of all living organisms • Uses: • Sequence Analysis & Comparison • Computational Evolutionary Biology • Genetics of Disease: Breast cancer, Alzheimer’s • Oncogenomics: Analysis of mutations in cancer • Analysis of causes of epidemics

  6. Cloning • Production of genetically identical populations of existing organisms, cells or tissues • Important Uses: • Cloning Stem Cells • Cloning whole organisms • Cloning of Extinct & Endangered Species • Some organisms that have been cloned: • Tadpoles, Carp, mice, sheep, pig, cattle, cat, dog, wolf, camel, Pyrenean Ibex

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