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Allyltriphenylphosphonium Bromide

Allyltriphenylphosphonium Bromide. Physical Properties Colorless to slight yellow liquid with an unpleasant odor Boiling Point: 70 ° C Melting Point: -119° C Density: 1.43g/cm^-3 Solubility: Negligible Chemical Properties Stable. Flammable. Incompatible with strong oxidizing agents

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Allyltriphenylphosphonium Bromide

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  1. Allyltriphenylphosphonium Bromide • Physical Properties • Colorless to slight yellow liquid with an unpleasant odor • Boiling Point: 70° C • Melting Point: -119° C • Density: 1.43g/cm^-3 • Solubility: Negligible • Chemical Properties • Stable. Flammable. Incompatible with strong oxidizing agents • Toxicology • Harmful if swallowed or inhaled • May be harmful if contact is made with skin • Liquid may burn skin or eyes • IPR-MUS LD50 1087 mg kg^-1 • IHL-RAT LC50 10g/m3/30m

  2. Melting Point Data Analysis Untreated Salmon DNA

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  4. Electrophoresis Results 6000 BP (1st band of both uncuts) 3800 BP (2nd band of both uncuts) 5000 BP (All 5 linearized, at 0, 10, 15, 20, and 30 ul of chemical)

  5. Electrophoresis Band Data • 600 Da (g/mol) is equal to the molecular weight of one DNA base pair (average) • Linear not treated and linear treated: 5,000 base pairs – Molecular weight = 3,000,000 Da • Large fragments (1st band) of uncut treated and not treated: 6,000 base pairs – Molecular weight = 3,600,000 Da • Small fragments (2nd band) of uncut treated and not treated: 3,800 base pairs – Molecular weight = 2,280,000 Da

  6. Before Optimization:Energy = 6537.1524 kcal/mol (Gradient = 218.231380)After Optimization:Energy = 237.824937 kcal/mol Before Optimization: Energy = 1704.619571 kcal/mol (Gradient = 278.200184) After Optimization: Energy = -114.977091 kcal/mol

  7. Discussion • The energy drop determined in Hyperchem indicated an interaction between the molecule and DNA. • This interaction was supported by a 10 degree Celsius shift of the melting point of DNA using the SmartSpec. • However, the electrophoresis did not directly support this theory. Our first test with electrophoresis did not yield any evidence of interaction between the chemical and DNA. We modified the experiment by testing various concentrations of our chemical to determine whether quantitative differences affect how the molecule interacts with DNA. The results of the second run also showed no evidence of the molecule binding with the DNA regardless of concentration or whether the DNA was uncut or linearized. • Based on Hyperchem predictions the molecule in its lowest energy state had a closest distance of 3 angstroms which is the distance of the hydrogen bonds between base pairs. Therefore our molecule should have been close enough for bonds to form. This leaves an open question as to what the interaction was or that our methodology for electrophoresis has flaws. • Our results suggest that more studies are needed to determine interactions of our molecule with DNA.

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