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sh RBFOX1. Figure_SupFig1. nt . A. B . RBFOX1. sh RBFOX1. shGFP. C. β-actin. RBFOX1. AVG 1.00 0.17 SEM 0.34 0.14. β-actin. AVG 0.76 1.00 0.38 SEM 0.17 0.11 0.08 Pval 0.32 - 1x10 -4. Supplemental Figure 1. RNA-interference knockdown of RBFOX1 .
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shRBFOX1 Figure_SupFig1 nt A. B. RBFOX1 shRBFOX1 shGFP C β-actin RBFOX1 AVG 1.00 0.17 SEM 0.34 0.14 β-actin AVG 0.76 1.00 0.38 SEM 0.17 0.11 0.08 Pval 0.32 - 1x10-4 Supplemental Figure 1. RNA-interference knockdown of RBFOX1. A) HeLa cells were transfected with vectors expressing HA epitope-tagged RBFOX1 and either a non-targeting scrambled short RNA hairpin (nt) or a hairpin against RBFOX1 exon 9 (shRBFOX1). Immunoblotting was performed using HA antibody and quantitation was performed by densitometry with normalization to β-actin. Average (AVG) and standard error of the mean (SEM) from two independent experiments are shown. B) PHNP cells were transduced with lentiviral constructs expressing either a non-targeting hairpin (shGFP) or the shRBFOX1 hairpin. Comparison is also made to non-transduced wild-type PHNP control cells (C). Quantitation was performed by densitometry with normalization to β-actin. Average (AVG) and standard error of the mean (SEM) are shown for three blots with five biological replicates each. Student’s t-test was used as a test of significance and the resulting p-value is shown. Representative images are shown.
