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5’- GTA CAG TGC AAT T AC GCC AGC ATG GCA AGA CAT AAT CAA C-3’

Alexa Fluor 488. Bgli site. 5’- GTA CAG TGC AAT T AC GCC AGC ATG GCA AGA CAT AAT CAA C-3’ 3’- CAT GTC ACG TTA ATG CGG TCG TAC CGT T CT GTA TTA GTT G-5’. Texas Red. Δ t >> t R separate molecules. photon counts. time. Δ t << t R same molecule. # events. E. Intensity (arb. units).

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5’- GTA CAG TGC AAT T AC GCC AGC ATG GCA AGA CAT AAT CAA C-3’

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  1. Alexa Fluor 488 Bgli site 5’-GTA CAG TGC AAT TAC GCC AGC ATG GCA AGA CAT AAT CAA C-3’ 3’- CAT GTC ACG TTA ATG CGG TCG TAC CGTTCT GTA TTA GTT G-5’ Texas Red

  2. Δt >> tR separate molecules photon counts time Δt << tR same molecule

  3. # events E

  4. Intensity (arb. units) Intensity (arb. units) -.5 0 +.5 -.5 0 +.5 -1 0 +1 -1 0 +1 r (m) r (m) z (m) z (m)

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