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Pakistan barcode project: progress and applications

Pakistan barcode project: progress and applications. Muhammad Ashfaq. 2010: Barcoding economically important insect species from Pakistan. Biodiversity Institute of Ontatio. National Institute for Biotechnology and Genetic Engineering (NIBGE). Funded by: HEC. Project execution.

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Pakistan barcode project: progress and applications

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  1. Pakistan barcode project:progress and applications Muhammad Ashfaq

  2. 2010: Barcoding economically important insect species from Pakistan Biodiversity Institute of Ontatio National Institute for Biotechnology and Genetic Engineering (NIBGE) Funded by: HEC

  3. Project execution

  4. Activities and Progress Shaking onto sheets Light traps Arial nets Manual catching Pipettes and sieves Collecting animal parasites

  5. Specimen preparation and databasing

  6. Specimens Animals: 34103 Plants: 1289 Barcodes Animals:28605 Plants: 988 18 months IDRC (62%)

  7. Beneficials Pests

  8. II. Plants: 363 Singletons: 1530 Number of BINs/ species Pakistan-only BINs/ species

  9. II. Genera: 698 Unidentified: 22308 III. Species: 764 Taxonomic identifications

  10. Barcode Applications

  11. Reference libraries

  12. Helicoverpa armigera 98 33 Helicoverpa zea (GU087831) Heliothinae Heliothis virescens (GU336572) 73 Masalia sp. Heliothis sp. 21 24 Heliocheilus confertissima 21 Spodoptera littoralis 90 Spodoptera litura Amphipyrinae 23 Spodoptera cilium 90 Spodoptera exigua 53 Mamestra sp. Mythimna sp. Hadeninae 19 5 Leucania venalba 18 Mythimna convecta 41 Agrotis segetum 92 Noctuinae Agrotis ipsilon Chrysodeixis acuta 2 49 Thysanoplusia orichalcea Plusiinae 91 Chrysodeixis eriosoma 41 Ctenoplusia albostriata 30 Trichoplusia ni 29 Hypocala deflorata Catocalinae Pandesma robusta 86 Dysgonia torrida 72 Achaea janata 89 Earias insulana Chloephorinae Earias vittella 99 Gelechiidae Pectinophora gossypiella Noctuid species in/around cotton fields of Punjab Previously only S. litura was reported from cotton in Punjab

  13. Genetic diversity: mango mealybug, Drosichamangiferae

  14. 91 Bt - 1 (36) [AAM1247] Asia II 1 99 40 Bt - 2 (1) [ AAM1246] 84 Bt - 3 (2) [ACB7718] 29 99 Bt - 4 (5) [ AAM1248] Asia II 7 63 99 Bt - 5 (2) 98 58 Bt - 6 (8) [AAM1243] 99 52 Bt - 7 (11) [AAM1245] Asia II 5 99 Bt - 8 (15) [AAM1244] Asia 1 99 99 Bt - 9 (2) 59 Bt - 10 (2) [AAA4495] 99 99 Bt - 11 (13) [AAT8875] MEAM1 97 Bt - 12 (19) 99 0.02 Bemisia tabaci, a complex of cryptic species

  15. Bemisia tabaci, distribution analysis (A) Asia II 1 (B) MEAM 1 (C) Asia 1

  16. Dengue epidemic in Punjab

  17. Mosquito species in Punjab

  18. Genus-wise distribution of mosquitoes in Punjab Genus: Aedes Aedes aegypti Aedes albopictus Genus: Anopheles Genus: Culex

  19. Outreach

  20. Barcoding project at NIBGE

  21. Barcoding team members

  22. Thank you for your attention

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