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BIO 201 Lab 12 Exp. 16: Week 2 Unknowns

BIO 201 Lab 12 Exp. 16: Week 2 Unknowns. Professor Diane Hilker. Overview. Exp. 16: Identification of Unknown Microorganisms. I. Exp. 16: Identification of Unknown Microorganisms. TODAY: Interpret Temp. Study (Exp. 7-Table1) Determine Optimum Temp.

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BIO 201 Lab 12 Exp. 16: Week 2 Unknowns

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  1. BIO 201 Lab 12Exp. 16: Week 2 Unknowns Professor Diane Hilker

  2. Overview • Exp. 16: Identification of Unknown Microorganisms

  3. I. Exp. 16: Identification of Unknown Microorganisms • TODAY: • Interpret Temp. Study (Exp. 7-Table1) • Determine Optimum Temp. • Any contaminants in tubes? • Observe/Describe Colony Morphology Plate • Repeat Gram Stain • Does it agree with last week’s results? • If need be, repeat Wet Mount & Simple Stain • Set up the necessary tests for YOU! • Incubate at YOUR Optimum Temp.

  4. I. Exp. 16: Identification of Unknown Microorganisms • Yeast • Opt. Temp: 5, 25, 37oC • Dry,white, crusty growth • Wet Mount: large, ovoid, irregular, budding, Brownian Movement • Simple Stain:large, ovoid, irregular, budding • Gram Stain: Same as above, cells stain either purple, pink or both • Catalase: + (bubbles) • No other testing necessary

  5. I. Exp. 16: Identification of Unknown Microorganisms • Gram Pos. Cocci: Staph and Strep • Opt. Temp: 25-37oC • Small white creamy colonies Staphylococcus • Gram Pos. cocci in clusters • Catalase: + • Halophile

  6. I. Exp. 16: Identification of Unknown Microorganisms Streptococcus • Gram Pos. cocci in chains • Catalase: - • Not a halophile • Today: 1. Do Catalase test (Exp. 15) 2. Inoculate Salt Plate (Staph 110 or MSA)

  7. I. Exp. 16: Identification of Unknown Microorganisms • Gram Pos. Rod: Bacillus stearothermophilus • Opt. Temp: 60oC • Transparent beige colonies • Simple Stain: endospores • Gram Pos. long rods. • Cell wall breaks down quickly & looks pink if not observed in 24 hrs.

  8. I. Exp. 16: Identification of Unknown Microorganisms • Gram Pos. Rod: Bacillus stearothermophilus • Today: • Inoculate Starch Plate (Exp. 15): dab the sample in the center of the plate • Incubate at 60oC in sealed plastic bag

  9. I. Exp. 16: Identification of Unknown Microorganisms • Gram Negative Rods: short rods • Opt. Temp: 25 -37oC • Beige large colonies, may be motile • E. coli • Pseudomonas-may have a little growth at 5oC, yellow-green pigmentation of media • Serratia- pink at 25oC • Enterobacter • Citrobacter • Proteus

  10. I. Exp. 16: Identification of Unknown Microorganisms • Gram Negative Rods • Today-Inoculate ALL of the following tubes (Exp. 15): • PRLB • PRDB • Tryptone Broth • MRVP Broth • Urea Broth • Simmons Citrate Slant • TSI Slant-Stab the bottom of the tube with inoculating needle

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