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Manganese Oxidizing Bacteria

Manganese Oxidizing Bacteria. Cynthia McClain HMC 2012 July 19, 2012. Mn(II ) Mn(III,IV ) Soluble. Mn-oxide/oxyhydroxide precipitate. Most MnOx in nature formed biotically ! . Niche. Physical and chemical gradients. Manganese Oxidizing Bacteria.

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Manganese Oxidizing Bacteria

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  1. Manganese Oxidizing Bacteria Cynthia McClain HMC 2012 July 19, 2012

  2. Mn(II) Mn(III,IV) Soluble Mn-oxide/oxyhydroxide precipitate Most MnOx in nature formed biotically!

  3. Niche Physical and chemical gradients

  4. Manganese Oxidizing Bacteria • Multicopperoxidase or peroxidase proteins

  5. Isolation Collect water or sediment sample (slurry) Streak 100-200µl supernatant on K media plate: MnCl2, peptone, yeast extract Incubate 1-3 weeks Restreak brown colonies Test with Leucoberbelin Blue (LBB)

  6. Experimental Results • Different morphologies • Color • Size • Positive LBB • Soil • Sand from mouth of Elkhorn Slough (2) • Kirby Park oily sheen (1) • Baby seal beach sand (1)

  7. Movie of LBB Test • Sand from mouth of Elkhorn Slough

  8. Soil

  9. Soil

  10. Kirby Park Oily Sheen

  11. Kirby Park Oily Sheen

  12. Baby Seal Beach

  13. Baby Seal Beach

  14. Manganese Oxidizing Bacteria • Phylogeny • Why oxidize Mn? • Environmental Significance

  15. Thank you!

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