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Mojca Brložnik 1 , Tomaž Curk 2 , Klaus Natter 3 , Uroš Petrovič 1

IDENTIFICATION OF GENETIC VARIANTS RESPONSIBLE FOR BIOTECHNOLOGICALLY IMPORTANT TRAITS AND THE DESIGN OF NEW GENERATION INDUSTRIAL YEAST STRAINS. Mojca Brložnik 1 , Tomaž Curk 2 , Klaus Natter 3 , Uroš Petrovič 1 1 Jožef Stefan Institute, Ljubljana, Slovenia.

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Mojca Brložnik 1 , Tomaž Curk 2 , Klaus Natter 3 , Uroš Petrovič 1

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  1. IDENTIFICATION OF GENETIC VARIANTS RESPONSIBLE FOR BIOTECHNOLOGICALLY IMPORTANT TRAITS AND THE DESIGN OF NEW GENERATION INDUSTRIAL YEAST STRAINS Mojca Brložnik1, Tomaž Curk2, Klaus Natter3, Uroš Petrovič1 1 Jožef Stefan Institute, Ljubljana, Slovenia. 2 University of Ljubljana, Slovenia. 3 University of Graz, Austria.

  2. X Skinny parent Fat parent

  3. Average population Extreme population Segregants Tiling microarray Identify the relevant QTLs (SNVs) in pooled strains. Sequencing Average population Extreme population QTL#1 position position

  4. Extreme population Average population QTL#1 Sequencing Tiling microarray position position Fat parent Candidate causative gene Skinny parent

  5. Fat parent Single swaps Candidate causative genes: Double swaps Triple swaps Quadruple swap

  6. CONCLUSIONS Robust strain, low productivity RANDOM MUTAGENESIS Hundreds - thousands mutants ALLELE SWAPS Identification of productivity-related mutations Robust strain, high productivity Non-robust strain,high productivity NEW GENERATION INDUSTRIAL MICROORGANISMS

  7. ACKNOWLEDGEMENTS Klaus Natter Yeast Genetics and Molecular Biology Group Institute of Molecular Biosciences University of Graz Austria Tomaž Curk Bioinformatics Laboratory Faculty of Computer and Information Science University of Ljubljana Slovenia

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