1 / 39

Using model organisms to study people

Fugu. Mouse. budding yeast fission yeast (6000 genes). Using model organisms to study people. (30,000 - 40,000 genes). “the worm” (19,000). “the fly” (12,000 genes). poly A 6 N 4. + 5 bp.

spence
Download Presentation

Using model organisms to study people

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. Fugu Mouse budding yeast fission yeast (6000 genes) Using model organisms to study people (30,000 - 40,000 genes) “the worm” (19,000) “the fly” (12,000 genes)

  2. poly A6N4 + 5 bp http://biology.uoregon.edu/Biology_WWW/Biospheres/winter95/D.Keller/Keller.html

  3. http://www.biokem.uu.se/Images/cap_dna.gif

  4. http://biology.kenyon.edu/courses/biol14/dna/chrom2.gif

  5. http://images.google.com/imgres?imgurl=www.biozentrum.unibas.ch/~izmb/atlas/htm/fig/fig53.jpg&imgrefurl=http://www.biozentrum.unibas.ch/~izmb/atlas/htm/p53.htm&h=510&w=412&prev=/images%3Fq%3Dbacterial%2Bdna%2Bspread%2B%2B%26svnum%3D10%26hl%3Den%26lr%3D%26ie%3DUTF-8%26sa%3DGhttp://images.google.com/imgres?imgurl=www.biozentrum.unibas.ch/~izmb/atlas/htm/fig/fig53.jpg&imgrefurl=http://www.biozentrum.unibas.ch/~izmb/atlas/htm/p53.htm&h=510&w=412&prev=/images%3Fq%3Dbacterial%2Bdna%2Bspread%2B%2B%26svnum%3D10%26hl%3Den%26lr%3D%26ie%3DUTF-8%26sa%3DG

  6. Chromosome-separating (pulsed field) gel electrophoresis

  7. Pulsed-field or CHEF gels separate megabase-sized DNAs

  8. Propidium iodide

  9. Ethidium bromide Intercalating dye

  10. Labelling DNA 32P enzymatically at the end (kinase) nick-translation (fill-in DNA synthesis) Non-radioactive fluorescent dyes

  11. 8-Fluo-cGMP

  12. SKY spectral karyotyping

  13. FISH (fluorescent in-situ hybridization) http://www.chrombios.com/WebFinals/PictureGal/Bilder/MausPanc2.jpeg http://www.nisiq.net/~dakoj/ish/telomere.gif

  14. http://images.google.com/imgres?imgurl=www.molecularprobes.com/lit/fish/images/g000448.gif&imgrefurl=http://www.molecularprobes.com/lit/fish/g000448.html&h=235&w=260&prev=/images%3Fq%3Dfish%2Bcentromere%26svnum%3D10%26hl%3Den%26lr%3D%26ie%3DUTF-8http://images.google.com/imgres?imgurl=www.molecularprobes.com/lit/fish/images/g000448.gif&imgrefurl=http://www.molecularprobes.com/lit/fish/g000448.html&h=235&w=260&prev=/images%3Fq%3Dfish%2Bcentromere%26svnum%3D10%26hl%3Den%26lr%3D%26ie%3DUTF-8

  15. www.pasteur.fr/recherche/unites/biophyadn/e-Fmapping.html

  16. http://escience.ws/b572/L1/L1.htm

  17. (2N3ATP[g]flouresceinamine 2N3ATP[g]rhodamine

  18. http://www.jcb.org/cgi/content/full/157/4/579/FIG2

More Related