1 / 1

spiro
Download Presentation

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. Altered biological activity of peroxynitrite-modified nerve growth factor (NGF) compared to native NGF in vitro. A: Dorsal root ganglia (DRG) neurons that are neurofilament-immunoreactive by immunofluorescence at 4 DIV (upper panel) and 28 DIV (lower panel) treated with native NGF (left) or peroxynitrite-modified NGF (right), 25X, Bar = 50 μm. B, C: Peroxynitrite-modified NGF treatment caused a reduction in cell soma of DRG neurons expressed as mean cross-sectional area (μm2). B: 4 DIV (n=30, P< 0.001); C: 28 DIV (n=30, P< 0.05); there was also a marked reduction in neural processes treated at both time-points. (D) Reduction in DRG cell survival (6 fields of 0.39 mm2/condition) in peroxynitrite-modified NGF at 4 DIV (P< 0.001). At 28 DIV, there was not significant reduction in cell number between native NGF vs. peroxynitrite-modified NGF, indicating that nitrated NGF loses capacity for maintaining the survival of newly cultured DRG neurons or maintaining the phenotype of mature and fully developed DRG cultured neurons.

More Related