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Y Y Y Y Y. Y Oe Y Oe Y Oe Y Oe Y Oe. Y Oe Y Oe Y Oe Y Oe Y Oe. Y O Y O Y O Y O Y O. Production of clean seed. The virus-free program at WRPIS (Pullman, WA).
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Y Y Y YY YOe YOe YOe YOe YOe YOe YOe YOe YOe YOe YO YO YO YO YO Production of clean seed The virus-free program at WRPIS (Pullman, WA)
History • 1983 >70% infected w/ BCMV • 1985 original protocol • 1994 leaf tissue testing • 23.3% of collection virus-free
The “TEST” • Tissue harvest • ELISA testing • Clean • “Dirty”
The “clean up” • <10% BCMV infection • rouging • >10% BCMV infection • single planting, harvest & testing • Re-combining clean seed
¿ The future ? • Widely recognized protocol • Adapted by other germplasm collections • Virus testing technician • Part-time help? Soft money? • Suggestions?????