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Cell signaling Part II Sit back, relax, and enjoy the show.

Cell signaling Part II Sit back, relax, and enjoy the show. Role of G-protein in Cellular Signaling. Biological Function - Smell &Taste (~1000 receptors) - light - Neurotransmitters - Chemotoxin - Exocytosis - Blood pressure - Embryogenesis - cell growth & differentiation

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Cell signaling Part II Sit back, relax, and enjoy the show.

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  1. Cell signaling Part II Sit back, relax, and enjoy the show.

  2. Role of G-protein in Cellular Signaling Biological Function - Smell &Taste (~1000 receptors) - light - Neurotransmitters - Chemotoxin - Exocytosis - Blood pressure - Embryogenesis - cell growth & differentiation - HIV infection - Oncogenesis Effectors of bg a • Ion channels • PI3Kg • PLC-b • adenylyl cyclases G12 GS Gq Gi cAMP K+ channels Na+/K+ exchange regulation Ca2+ channels cAMP PLC-b1-3 Na+ channels PLC, cGMP

  3. The activated receptor has GEF activity G nucleotide Exchange Factor Galpha-GTP is active and can Act on effectors The intrinsic GTPase activity returns the G protein complex to its resting state.

  4. Hormone activation and inhibition of adenyl cyclase is mediated by Gs and Gi

  5. Cholera toxin knocks out the GTPase activity so that there is constant AC stimulation. This opens channels to permit water flow into the intestine Pertusis toxin (Whopping Cough) blocks the GDP of Gi so that there is no inhibition of AC. Vibrio cholera Bordetella perusis

  6. Bbg does it too

  7. So what? What can G-protein-coupled signaling do?

  8. PKA, for those who want to know Modulatory synapse 3 neurons talking Glutamate is also a neurotransmitter

  9. Wow man, that’s the real stuff Serotonin is your friend

  10. Now for something completely different: vision. Well, not really that different. How is light absorbed? How is the signal transduced? How do rod cells adapt to large variations in light intensity?

  11. Direction of light into your eye

  12. 800 eyes, ommatidia 22 cells each 8 neurons, retinula, R cells RTK, Sevenless BOSS, Bride of Sevenless Constitutively active Ras rescues

  13. Activation means recruitment Proximity = action

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