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VIBRIONACEAE

VIBRIONACEAE. It contains 3 genera: Vibrio Aeromonas Plesiomonas VIBRIO: GENERAL CHARACTERS : Gram negative, rigid, curved rods. vibratory motility – charecteristic feature

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VIBRIONACEAE

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  1. VIBRIONACEAE

  2. It contains 3 genera: Vibrio Aeromonas Plesiomonas • VIBRIO: • GENERAL CHARACTERS: Gram negative, rigid, curved rods. vibratory motility – charecteristic feature asporogenous, non-capsulated

  3. VIBRIO SPECIES: V.cholerae V. mimicus HALOPHILIC VIBRIOS: V.parahaemolyticus V.alginolyticus V. vulnificus

  4. GARDNER AND VENKATRAMAN’S CLASSIFICATION: VIBRIO GROUP A GROUP B (biochemically and antigenically heterogenous ) OSUB GROUP O1 Non-01 (SERO GROUPS) (up to 0-13) (SERO VARS) BIOTYPES : CLASSICAL El Tor SEROTYPES : Ogawa Inaba Hikojima

  5. TEST CLASSICAL El Tor • Hemolysis _ + • VP test _ + • Chick erythrocyte _ + agglutination • polymyxinB sensitivity + _ • GroupIV phage Susceptibility + _ • El Tor phage V Suceptibility _ +

  6. VIBRIO CHOLERAE MORPHOLOGY • Short ,curved ,cylindrical rods • V.comma • S-shaped or spiral forms • Pleomorphism • Fish in stream appearance • Darting motility • Swarm of gnats

  7. CULTURAL CHARACTERISTICS • Strongly aerobic • Temp 16-40 C,PH :6.4-9.6 • Requirement of Nacl • Grows well on ordinary media • Nutrient agar: moist,translucent,rounddiscs,1-2mm in diameter with bluish tinge in transmitted light

  8. Mac conkeys agar:- Late lactose fermenters Blood agar:- hemodigestion Gelatin stab culture:- infundibuliform or napiform liquefaction Peptone water:- forms surface pellicle-in 6hrs

  9. SPECIAL MEDIA:- 1.Holding or transport media:- a.Venkatraman-Ramakrishnan medium b.Cary-Blair medium c.Autoclaved sea water 2.Enrichment media:- a.Alkaline peptone water b.Monsur’staurocholatetellurite peptone water 3.Plating media:- a.alkaline bile salt agar b.monsur’s gelatin taurocholatetrypticase tellurite agar c.TCBS medium

  10. BIOCHEMICAL REACTIONS:- catalase -positive oxidase - positive Indole - positive methyl red -negative voges-proskauer test -negative citrate test - positive nitrate test - positive

  11. Sugar fermentation tests ferments glucose mannitol maltose mannose sucrose with the production of acid only not ferments inositol arabinose or lactose • Vibriosdecarboxylate lysine and ornithine but not arginine • Vibrios elaborate several enzymes • CHOLERA RED REACTION

  12. RESISTANCE • Susceptible to heat ,drying and acids & disinfectants • Resist high alkalinity • Destroyed at 55 C in 15 min • Affected by vibriophages • Killed in the gastric juice of normal acidity with in min • El Tor vibrios survive longer than classical cholera vibrio

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