1 / 16

Applications of Analytical Chemistry at RTI International

Applications of Analytical Chemistry at RTI International. Argon plasma instruments Atomic Emission (ICP-AES) – 3 Quadrupole Mass Spectrometers (ICP-MS) - 2 Sector Field Mass Spectrometer (SFICP-MS) -1 Fluorescence Cold Vapor (for Hg) Hydride Generation (for As, Se, etc.) Absorption.

stamos
Download Presentation

Applications of Analytical Chemistry at RTI International

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. Applications of Analytical Chemistryat RTI International • Argon plasma instruments Atomic Emission (ICP-AES) – 3 Quadrupole Mass Spectrometers (ICP-MS) - 2 Sector Field Mass Spectrometer (SFICP-MS) -1 • Fluorescence Cold Vapor (for Hg) Hydride Generation (for As, Se, etc.) • Absorption

  2. Contamination Control • Class 10, 100 hoods • Class 100 cleanrooms • Class 10,000 cleanroom

  3. Analytical Support RTI has performed major component, trace and ultra-trace level organic & inorganic analyses in support of variety of studies… • Animal toxicology/ADME studies - Bulk chemical characterization, impurity analysis, tissue burden analysis. • Human exposure studies - (Hg in hair; Pb in blood; As, Cd, Se, Cu, etc. in blood and plasma, etc.) • Environmental studies – drinking water, food, air samples, sediments, etc.

  4. Atomic Spectrometry at RTI International Essential Trace Metals: Cr, Mn, Fe, Co, Ni, Cu, Zn, Mo, Se…?

  5. Assessment of Human Exposure to Arsenic • Exposure: • Ingestion - Drinking water and food. • Inhalation – negligible • Internal dose: • Urine analysis • Hair analysis • Toxicity of Species: • As(III), As(V), AsB, AsC, MMA, and DMA

  6. Interferences – Atomic Masses • High-Resolution ICP-MS solves many problems… • …but is not a magic bullet As=74.921594 ArCl=39.962384+34.965903=74.928287 As As 74.9150 Mass Resolution 74.9670 73.9216 Mass Resolution 75.9605

  7. Isobaric Interference on 75As

  8. Chemical Form: Arsenic

  9. Calibration Standard – Urine matrix • Chromatogram 10ppb calibration standard (urine). • Key: • 1- AsB, 2 - As3+ • 3-DMA, 4-AsC • 5-MMA, 6-As5+ • IS - Internal standard (Potassium Hexahydroxy Antimonate V).

  10. Urine sample. 1-AsB, 2-DMA, and 3-MMA.

  11. North Carolina Stream Data Data from North Carolina Geological Survey – ca. 1993

  12. L-ephedrine • Mol wt.: 165.23 • Mol formula: C10H15NO Analytical work: • Identification/Confirmation • Impurity determination

  13. Identity - FTIR Assignments: 3470-N-H Stretch; 3298-N-H Stretch; 3085-O-H Stretch; 2979- Aromatic C‑H Stretch; 1491-N‑CH3 Bend; 1450-N‑CH3 Bend; 1373-N‑CH3 Bend; 1353-C‑O Stretch; 1136-C‑N Stretch; 1089-O‑H Stretch; 983-C‑H Bend; 750-Aromatic C‑H Bend; 702-Aromatic C‑H Bend.

  14. NMR spectrum

  15. Identity - NMR

  16. Identity/Purity - HPLC LC analysis: compare with standards. Peak No. Ret. Time (min) Rel. Ret. Time % of Total Area 1 15.19 1 100

More Related