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Jesper Bartholin Bruhn,1* Allan Beck Christensen,2 Lars Ravn Flodgaard,1

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, July 2004, p. 4293–4302 Vol. 70, No. 7. Presence of Acylated Homoserine Lactones (AHLs) and AHL-Producing Bacteria in Meat and Potential Role of AHL in Spoilage of Meat.

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Jesper Bartholin Bruhn,1* Allan Beck Christensen,2 Lars Ravn Flodgaard,1

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  1. APPLIED AND ENVIRONMENTAL MICROBIOLOGY, July 2004, p. 4293–4302 Vol. 70, No. 7 Presence of Acylated Homoserine Lactones (AHLs) andAHL-Producing Bacteria in Meat and PotentialRole of AHL in Spoilage of Meat Jesper Bartholin Bruhn,1* Allan Beck Christensen,2 Lars Ravn Flodgaard,1 Kristian Fog Nielsen,3 Thomas Ostenfeld Larsen,3 Michael Givskov,2 and Lone Gram1 Received 26 September 2003/Accepted 8 March 2004 學生:吳俊昌

  2. Introduction • Enterobacteriaceae 腸道菌 • 種類 例: Hafnia alvei 718 腐肉中最多 Serratia spp. 次多 Serratia proteamaculans B5a 產protease使牛奶coagulation

  3. Introduction • Monitor bacteria Agrobacterium tumefaciens NT1(pZLR4) AHL-reporter plasmid 偵測有oxo,6-14C Chromobacterium violaceum CV026 偵測無oxo,4C

  4. Introduction • AHL有不同的structure 例: N-3-oxo-hexanoyl homoserine lactone(OHHL) 腐肉中佔最多 比沒有oxo的利用率,效能及持久度高 N-hexanoyl homoserine lactone(HHL) 腐肉中次多 N-octanoyl homoserine lactone(OHL)

  5. 菌數濃度

  6. 分離菌種

  7. 主要的AHL構型 1.Marker 2.H. alvei 718 3.4 week再萃取 4.3 week (fat) 5.2 week (fat) 6.1 week (low fat) 7.當天 (fat) 利用ESI+-MS再度證實

  8. AHL影響phenotype 1.marker 2.718 3.718 4.718halⅠmutant 5.718halⅠmutant 6.718halⅠmutant + OHHL 7.718halⅠmutant + OHHL 8.marker 20kDa

  9. Bacteria communication

  10. Summary • Hafnia alvei 718 為主要 • 以N-3-oxo-hexanoyl homoserine lactone(OHHL)為主要構型 • 718 halⅠmutant影響phenotype • Bacteria communication(718 and B5a)

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