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  1. Figure S2. Sequence alignment of GSI-like portion of Arabidopsis NodGS polypeptide with GSI-like part of FluG from Aspergillus nidulans, GSI-like protein of Medicago truncatula (Mtr), and selected bacterial GSI enzymes from Bacillus pumilus (Bpu), Clostridium leptum (Cle), Geobacillus kaustophilus (Gka), Lactobacillus casei (Lca), Methanococcus voltae (Mvo), Pseudomonas fluorescens (Pfl), Streptomyces ambofaciens (Sam), and Salmonella typhimurium (Sty). Bar above the alignment indicates the amino acids in the S. typhimurinum GS involved in the active site.The chemical nature of the individual amino acids is color-coded as follows: cyan, W,F,V,Y,L,I,H,M,A; orange, G; yellow, P; magenta, D,E; green, N,S,T,Q; red, R,K; pink, C. Identical amino acids are marked with asterisk, conserved substitutions are marked with colon, and semi-conserved substitutions are marked with dot.

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