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Supplementary 1. AsPC-1 Colo-357. Control DN-Nrf2. -. -. +. +. -. -. +. +. Nrf2. GCLC. HO-1. b -actin.
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Supplementary 1 AsPC-1 Colo-357 Control DN-Nrf2 - - + + - - + + Nrf2 GCLC HO-1 b-actin Supplementary 1.Inhibition of Nrf2 activity by DN-Nrf2 vector was confirmed by measuring Nrf2 target gene products (e.g., HO-1). After 24 hr of transfection with control or DN-Nrf2 vector, cells were harvested, and total cell lysates were used for immunoblotting assay analysis as described in Material and Methods.