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enrichment of DTC in peripheral blood samples of 101 patients with transitional cell carcinoma of the bladder (bTCC); 208 blood samples (BS) before & after TUR-B, cystectomy (cys) density gradient centrifugation (Ficoll) by magnetic cells sorting (AutoMACS, Miltenyi Biotec)
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enrichment of DTC in peripheral blood samples of 101 patients with transitional cell carcinoma of the bladder (bTCC); • 208 blood samples(BS)before & after TUR-B, cystectomy (cys) • density gradient centrifugation (Ficoll) by magnetic cells sorting • (AutoMACS, Miltenyi Biotec) • depletion of leukocytes (CD45 microbeads) with • DTC in the negative fraction • Cytospin centrifugation and ICC (anti-CK-ab MNF116, Dako) • DTC detection & quantification by positive CK staining and patho-morphological assessment, FISH (UroVysion) for selected cases • detection of typical chromosomal alterations Detection of disseminated tumor cells (DTC) in the peripheral blood of bladder cancer patients for therapy assessment U. Schmidt1, A. Meye1Udo Bilkenroth2, Katja Robel1, S. Fuessel1, Clemens Linné1, Christian Höfling1, Oliver W. Hakenberg1, M.P. Wirth1 1Department of Urology, Technical University of Dresden, Germany; 2Department of Pathology, Martin-Luther-University of Halle, Germany
Results/Summary: • 58 of 208 blood samples (28 %) from 33 patients contained DTC • detection of DTC independent of tumor stage & grade • cells of bTCC disseminate early • less DTC after TUR-B & cystectomy compared to pre-operative BS • surgical intervention caused a clear reduction of DTC Detection of disseminated tumor cells (DTC) in the peripheral blood of bladder cancer patients for therapy assessment