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Onur Ergen

“Flexible electrical recording from cells using nanowire transistor arrays”. Onur Ergen. Electirical Engineering and computer science University of California, Berkeley onur@eecs.berkeley.edu. EE235 Student Presentation 2 4 may 2009. Outline.

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Onur Ergen

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  1. “Flexible electrical recording from cells using nanowire transistor arrays” Onur Ergen Electirical Engineering and computer science University of California, Berkeley onur@eecs.berkeley.edu EE235 Student Presentation 2 4 may 2009

  2. Outline • Advantage of using NWsfor establishing active interfaces with biological systems. • Fabrication and transfer method of NWs. • Discussion on experimental approach and electrical results of cardiomyocyte signals. • Conclusion

  3. Fabrication and transfer method of NWs Synthesis of semiconductor silicon nanowires. Scheme of nanowire synthesis set-up. Transfer of Nws with using contact printing. • Javey A, Nam S, Friedman RS, Yan H, Lieber CM (2007) Layer-by-layer assembly of nanowires for three-dimensional, multifunctional electronics. NanoLett 7:773–777. • PatolskyF, Zheng G, Lieber CM (2006) Fabricaton of silicon nanowire devices for ultrasensitive, label-free, real-time detection of biological and chemical species. Nat Protocols 1:1711–1724. A P

  4. Schematic of Experimental Approach • NWFET arrays coupled to embryonic chicken cardiomyocytes. • A-) NWFET chip, where NW devices are located at central region of chip. Source and 2 drain electrodes connected to a vertically oriented NW. • B-)Cardiomyocytes cultured on thin rectangular pieces of PDMS. • C-) PDMS piece with cultured cells oriented over the device region of NWFET chip. • D-)Schematic view of a cardiomyocyte oriented over a NW FET device. Embryonic chicken cardiomyocytes.

  5. Measurement of Cardiomyocyte Signals --Measurement of cardiomyocytesignals-- --Effect of applied force on recorded signals-- Back into contact retracted

  6. Previous Approach ; Electrical Recording from Hearts with Flexible NanowireDevice Arrays B.P. Timko, T. Cohen-Karni, G. Yu, Q. Qing, B. Tian and C.M. Lieber, "Electrical Recording from Hearts with Flexible Nanowire Device Arrays," NanoLett. 9, 914-918 (2009).

  7. Measurement of Cardiomyocyte Signals --Recording from Distinct Regions-- --Multiplexed Recording--

  8. Conclusions • Good flexible approach to interface silicon NWFETs with cells in which optically transparent PDMS/cell substrates are manipulated to place specific cells over particular NWFET elements. • Amplitude variations signals of cardiomyocytes from 31 to 7 nSfor different Vg values recorded (while keeping from calibrated voltage remained constant ). • Multiplexed recording of signals shows cardiomyocytemonolayers enabled temporal shifts and signal propagation. • Approach increases the flexibility and opens up opportunities for interfacing cardiomyocytes and other cells to high-performance NWFETs,

  9. Supplementary • Javey A, Nam S, Friedman RS, Yan H, Lieber CM (2007) Layer-by-layer assembly of nanowires for three-dimensional, multifunctional electronics. NanoLett 7:773–777.

  10. Transport measurement of SiNW devices in air Nws transfer on Si substrate by drop-cast • PatolskyF, Zheng G, Lieber CM (2006) Fabricaton of silicon nanowire devices for ultrasensitive, label-free, real-time detection of biological and chemical species. Nat Protocols 1:1711–1724. A P

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