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Introduction

Introduction

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Introduction

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  1. Introduction􀁡microscopy Introduction 􀁡microscopy 􀁡most cells are .01-.1 mm 􀁡unaided eye sees .1mm 􀁡Hooke 􀁠built microscope (30X)

  2. Purpose of Microscope 􀁡magnification 􀁠 enlarged image by a factor 􀁡resolving power 􀁠 ability to separate two adjacent points in a specimen 􀁡contrast 􀁠 how well details stand out against the background 􀁡parfocal 􀁠remain in focus when switching objectives

  3. Rules and Care of theMicroscope, abridged 􀁡carry microscope correctly 􀁡clean lenses with lens paper and slides with Kimwipes 􀁡set smallest objective in place (4X) and bring stage close of objectives 􀁡use oil with oil immersion lens (100X) only 􀁡clean all lenses when finished 􀁡have instructor check scope before storing

  4. The Compound Microscope • Metric measurement of a field of view • 􀁡ocular = 10X • 􀁡mag of lens total mag. (ocular x obj.) • 4X 40X • 10X 100X • 40X 400X • 100X 1000X

  5. 6 • Exercises • Ex. 7.1 fill in table • Ex. 7.2 depth of focus: • colored threads,determine order • Ex. 7.3 image orientation • letter “e” ; draw at 40x and 100x • 􀁡Ex. 7.4 dissecting microscope • 􀁠Observe bacterial culture dish

  6. Other Microscopes • 􀁡dissecting,greater working distance,reflected and transmitted light • 􀁡light microscope • 􀁡phase contrast microscope • 􀁡transmission electron microscope, TEM • 􀁡scanning electron microscope, SEM

  7. Cell Theory • Cell Theory • 􀁡all organisms are made of cells • 􀁡the cell is the smallest living thing • 􀁡all cells arise from pre-existing cells

  8. Eukaryotic Cells • Plant : Elodea cells w/ water • draw and label @ 400X total mag. • Label nucleus ,plasma membrane • cytoplasm w/ cytosol,cell wall of cellulose • Chloroplast,vacuole

  9. Eukaryotic Cells • Plant : Onion bulb cellsw/ iodine • 􀁡draw and label @ 400X total mag. • Label nucleus w/ DNA,plasma membrane • cytoplasm w/ cytosol,cell wall of cellulose • Nucleolus,vacuole

  10. Eukaryotic Cells • Animal : human cheek cells • w/ methylene blue stain • draw and label @ 400X total mag. • nucleus w/ DNA, plasma membrane,cytoplasm w/ cytosol

  11. Prokaryotic Cells • 􀁡label cell,cell wall of peptidoglycan • plasma membrane,cytoplasm w/ cytosol • Ribosome,nucleoid w/ DNA • draw three basic shapes • determine stain reaction • Gram positive = purple • Gram negative = red

  12. ModelsStudy both animal and plant cell models • 􀁡

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