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Supplementary Figure 4. ssDNA-positive cells (A, B) , TUNEL-positive cells (C, D), and histopathological changes (Klüver-Barrera stain) (E, F) in the mammillary body of PTD mice. B, D and F correspond to the rectangle areas in A, C and E, respectively. In contrast to TUNEL-positive cells, virtually no ssDNA-positive cells were observed in the mammillary bodies of PTD mice (B). Vacuolation of neuropil, vascular dilation, perivascular hemorrhage and loss of neurons were observed (E and F). Scale bar in (E) = (A, C, E) 100 μm. Scale bar in (F) = (B, D, F) 100 μm.