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The expected molecular weight for GST-Nup42 AA 1-382 is 67,144 Da

Purification of GST-Nups from E. coli via Ligand-Affinity Chromatography. The expected molecular weight for GST-Nup42 AA 1-382 is 67,144 Da. WCE. MSS. BSA. MW. MSS. kDa. +IPTG. 5 ul. 1ug. 2ug. 4ug. 50ul. 200. 116. 97. 68. BSA. GST-Nup42 AA 1-382. 44. 31.

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The expected molecular weight for GST-Nup42 AA 1-382 is 67,144 Da

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  1. Purification of GST-Nups from E. coli via Ligand-Affinity Chromatography The expected molecular weight for GST-Nup42 AA 1-382 is 67,144 Da WCE MSS BSA MW MSS kDa +IPTG 5 ul 1ug 2ug 4ug 50ul 200 116 97 68 BSA GST-Nup42 AA 1-382 44 31

  2. Affinity-Capture of Karyopherins from Yeast Lysates Using GST-Nups as Bait The expected molecular weight of GFP-Mex67 is 96 kDa The concentration of protein in the GFP-only HSS cytosol was [ ] MgCl2 eluates SDS eluates The concentration of protein in the GFP-Kap HSS cytosol was [ ] yeast HSS yeast HSS GFP GFP-Kap GFP-Kap GFP GFP-Kap GFP-Kap MW kDa 200 116 97 68 GST-Nup116 FG domain Kaps 44 GST-Nsp1 K>R 31

  3. Charting the path that karyopherins take across the nuclear pore complex Cytoplasm Nuclear Pore Complex Nup159 Nup159 Nup116h Nup116s Nup116h Nup116h Nup116s Nup42 Nup42 Nup100s Nup100h Nup100h Nup100s Nup57 Nup57 Nsp1s Nsp1s Nup49 Nsp1h Nsp1h ER lumen Nup49 Nsp1s Nsp1h Nsp1h Nsp1s Nup49 Nup49 Nup145h Nup145h Nup57 Nup145s Nup145s Nup57 Nup60 Nup60 Nup2 Nup2 Nucleoplasm Nup1h Nup1h Nup1s Nup1s [Turn the box green as shown, if your GFP-Kap bound to the FG domain in the BEAD HALO assay]

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