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* Corresponding author: s.gopalakrishnan@cgiar

Actinomycetes and their secondary metabolites to control multiple pathogens of chickpea and sorghum S. Gopalakrishnan* and GV Ranga Rao ICRISAT, Patancheru, Hyderabad, Andhra Pradesh, India. The list of herbal vermicomposts used in this study

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* Corresponding author: s.gopalakrishnan@cgiar

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  1. Actinomycetes and their secondary metabolites to control multiple pathogens of chickpea and sorghum S. Gopalakrishnan* and GV Ranga Rao ICRISAT, Patancheru, Hyderabad, Andhra Pradesh, India The list of herbal vermicomposts used in this study Jatropha curcas, Annona squamosa, Parthenium hysterophorus, Oryza sativa, Gliricidia sepium, Adhatoda vasica, Azadirachta indica, Capsicum annuum, Calotropis gigantea, Calotropis procera, Datura metal, Allium sativum, Zingiber officinale, Ipomoea batatas, Momordica charantia, Moringa oleifera, Argyranthemum frutescens, Nerium indicum, Allium cepa, Curcuma aromatica, Pongamia pinnata, Abacopteris multilineata, Nicotiana tabacum, Tridax procumbens and Vitex negundo INTRODUCTIONIn the present study, 25 different herbal vermicomposts were screened for actinomycetes that contain antifungal potential against Fusarium wilt and collar rot of chickpea (caused by Fusarium oxysporum f. sp. ciceri and Sclerotium rolfsii, respectively) and charcoal rot of sorghum (caused by Macrophomina phaseolina) and anti-insect potential against Helicoverpa armigera, the polyphagous pest of many crops. The promising ones were selected further for metabolite purification studies. • MATERIALS AND METHODS • Prominent actinomycetes isolated with SCA by spread plate methods. • In-vitro antifungal activity by dual-culture assay. • In-vivo anti-insect activity by bioassay (on 3rd instar Helicoverpa larvae) • Evaluated for PGP traits on respective selective media. • Actinomycetes identified by 16S rDNA analysis. • Metabolite production capability by solvent partitioning and bioassay. • Partial purification done by SPE and open column chromatography. The effect of culture filtrates of actinomycetes on FOC • RESULTS • Three most promising actinomycetes (CAI-21, CAI-26 and MMA-32) based on biocontrol and PGP traits were further studied • CAI-21 was identified as Streptomyces albus, while CAI-26 and MMA-32 were identified as S. champavatii and S. roseoviolaeus, respectively. • Culture filtrates of all the three actinomycetes completely inhibited FOC but not M. phaseolina completely. However, all the culture filtrates inhibited another pathogen of chickpea, Sclerotium rolfsii which causes collar rot. • Culture filtrates of all the actinomycetes showed 58-78% mortality over control. • Upon further fractionation of the culture filtrates of one of the actinomycetes (CAI-21) by open column chromatography, only 80% MeOH fraction showed inhibition of FOC. SRI CONT The effect of culture filtrates of actinomycetes on S. rolfsii Biocontrol and PGP traits of the actinomycetes The effect of culture filtrates of actinomycetes on H. armigera Molecular identification of the potential actinomycetes CA1-17 Control Fractionation of culture filtrates of CAI-21 on an open column chromatography: on FOC The effect of culture filtrate of CAI-21 on various pathogens On FOC On M. phaseolina On S. rolfsii *Corresponding author: s.gopalakrishnan@cgiar.org

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