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MALUS , THE BAD APPLE?. Preston K. Andrews Department of Horticulture and Landscape Architecture Washington State University USA. Ascorbate-glutathione cycle. Mehler-peroxidae rxn. GSSG. SOD. ASC. O 2 . H 2 O 2. NADPH. NAD(P). PS. APX. PSI. MDAR. DHAR. GR. NAD(P)H. O 2.
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MALUS, THE BAD APPLE? Preston K. Andrews Department of Horticulture and Landscape Architecture Washington State University USA
Ascorbate-glutathione cycle Mehler-peroxidae rxn GSSG SOD ASC O2. H2O2 NADPH NAD(P) PS APX PSI MDAR DHAR GR NAD(P)H O2 NADP H2O MDA GSH DHA MEHLER-PEROXIDASE CYCLE
TOMATO FRUIT MUTANTS Lycopersicon esculentum • Dark green dg; Chl persisting until maturity • High pigment hp; high Chl, carotenoids, AsA • Unpigmented epidermis y; colorless skin • Delta Del; increased -carotene, inhibited lycopene • Ghost +/gh; incomplete Chl deficiency • Ripening inhibitor rin; green fruit at maturity L. pimpinellifolium • Sunscald resistant sca • Superoxide dismutase Sod; isozyme 2
BIOCHEMICAL METHODS • Ascorbate - Ascorbate oxidase (Foyer et al., 1983) • Glutathione - Glutathione reductase/ 2-vinylpyridine (Griffith, 1980) • Chlorophyll & total carotenoids (Lancaster et al., 1994; Holden, 1976; Gross, 1991) • Anthocyanins (Francis, 1982)