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(classical) genetics

(classical) genetics. causality. DNA. phenotype. ‘reverse’ genetics. T. C. The ‘gapped duplex’ method of in vitro mutagenesis. Gapped duplex method with phenotypic selection. Ceftazidime. S K G mutant TEM bla

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(classical) genetics

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  1. (classical) genetics causality DNA phenotype ‘reverse’ genetics

  2. T C

  3. The ‘gapped duplex’ method of in vitro mutagenesis

  4. Gapped duplex method with phenotypic selection

  5. Ceftazidime

  6. S K G mutant TEM bla 5´ p GAT AAA TCT GGA GCC TCC AAG GGT GGG TCT CGC GG 3´ -----gat aaa tct gga gcc GGT GAG Cgt ggg tct cgc gg------ G E R wild-type TEM bla

  7. Target : replace Trp by Phe Screening aid : MnlI cleavage Mismatch primer : …GCCCTGGGCTTCGGTGGCA… Creating or removing a restriction recognition site as an aid in screening for site-specific mutants

  8. Codewoord tabel voor ‘reverse translation’ Leu = 1T2 Arg = 3G4 Ser = 567

  9. Ceftazidime is a third-generation cephalosporinantibiotic. Like other third-generation cephalosporins, it has broad spectrum activity against Gram-positive and Gram-negativebacteria. Unlike most third-generation agents, it is active against Pseudomonas aeruginosa, however it has weaker activity against Gram-positive microorganisms and is not used for such infections. Ceftazidime is a semisynthetic, broad-spectrum, beta-lactam antibiotic for parenteral administration. Ceftazidime is bactericidal in action exerting its effect by inhibition of enzymes responsible for cell-wall synthesis. A wide range of gram-negative organisms is susceptible to ceftazidime in vitro, including strains resistant to gentamicin and other aminoglycosides. In addition, ceftazidime has been shown to be active against gram-positive organisms. It is highly stable to most clinically important beta-lactamases, plasmid or chromosomal, which are produced by both gram-negative and gram-positive organisms and, consequently, is active against many strains resistant to ampicillin and other cephalosporins. Ceftazidime has activity against the gram-negative organisms Pseudomonas and Enterobacteriaceae. Its activity against Pseudomonas is a distinguishing feature of ceftazidime among the cephalosporins.

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