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Stopping Criteria : R 2 ≥ 0.60 0.80 < slope < 1.25

P3. P4. I 6. Hierarchical Transmission of changes in mRNA layer. I 2. Carbon Source A. B 2. I 1. I 4. B 1. I 5. I 3. 4 EFMs. P2. P1. Carbon Source B. P3. Stopping Criteria : R 2 ≥ 0.60 0.80 < slope < 1.25. I 6. I 2. B 2. I 4. B 1. I 5. I 3. P2. 3 EFMs. P1.

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Stopping Criteria : R 2 ≥ 0.60 0.80 < slope < 1.25

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  1. P3 P4 I6 Hierarchical Transmission of changes in mRNA layer I2 Carbon Source A B2 I1 I4 B1 I5 I3 4 EFMs P2 P1 Carbon Source B P3 Stopping Criteria: R2≥ 0.60 0.80 <slope < 1.25 I6 I2 B2 I4 B1 I5 I3 P2 3 EFMs P1 r =f([A],[B],[E]) r A B Flux, r Enzyme, [E] mRNA

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