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Transfer Disinfection Validation. Sarah Hiom Cardiff and Vale NHS Trust. Aseptic Preparation Isolator Technology. Transfer into controlled work zone. High contamination risk Disinfection (70% alcohol). Validation of Disinfection Transfer. Current methods Swabs/Contact plates
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Transfer Disinfection Validation Sarah Hiom Cardiff and Vale NHS Trust
Transfer into controlled work zone High contamination risk Disinfection (70% alcohol)
Validation of Disinfection Transfer • Current methods Swabs/Contact plates • Lack sensitivity Not appropriate for assessing disinfection
Aim • To develop and validate an accurate in house disinfection assessment method • To use this method to benchmark contamination levels and compare disinfection transfer procedures in pilot sites across Wales
Validate new method • Background count • Effect of residual alcohol on recovery rates • Determining % recovery
% Recovery S.epidermidis stock culture = 55cfu/100μl Contact Plate ~ 40% Swab ~ 20%
Benchmark Contamination Levels • 50ml glass vial • Batch (6) stored in unit for 5 days • Bioburden determined after each disinfection point during transfer to critical work zone (n 5)
Pilot conclusions • Novel sensitive method to assess disinfection transfers • Spray & wipe transfer methods significantly better at reducing bioburden than spray only procedures (P<0.1) • Better bioburden reduction when vial caps opened before alcohol spray disinfection transfers (P<0.1) • Operational procedures showed transfer of up to 9 cfu/vial into critical work zones
Action Limits “…for transfer disinfection procedures to pass this validation test, results are expected to demonstrate no greater than two colony forming units from each item sampled within the critical work zone. ” SteriVal : Technical Information Sheet 3.3