1 / 4

GLI 1

OCI-LY10 Cells. 293T Cells. 5. 4. 2^(-ddct). 3. Relative mRNA levels. 2. 1. 0. SMO. PTC. GLI 1. GLI 2. GLI 3. ABCG2. Supplementary Figure 1. 100. 80. % Viability. 60. 40. 20. 0. 0 0.05 0.1 1.0. Methotrexate (µg/mL). c. 48h. b. HS- 5 cells. HS- 5 cells. 48h.

talia
Download Presentation

GLI 1

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. OCI-LY10 Cells 293T Cells 5 4 2^(-ddct) 3 Relative mRNA levels 2 1 0 SMO PTC GLI 1 GLI 2 GLI 3 ABCG2 Supplementary Figure 1

  2. 100 80 % Viability 60 40 20 0 0 0.05 0.1 1.0 Methotrexate (µg/mL) c 48h b HS- 5 cells HS- 5 cells 48h 100 100 80 80 % Viability % Viability 60 60 40 40 20 20 0 0 Control FTC (10µM) Control Cyclopamine-KAAD (2µM) a 48h HS- 5 cells 100 80 % Viability 60 40 20 0 0 0.05 0.1 1.0 Doxorubicin (µg/mL) Supplementary Figure 2

  3. a 3.5 DLBCL cell lines 2.5 Relative ABCG2 mRNA [ 2^(-ddct)] 1.5 0.5 0 HBL-1 Pfeiffer OCI-LY3 OCI- BJAB DOHH2 Sudhl2 Sudhl4 U2940 MCF-7 Normal LY10 PBMC b c Cells Alone Co-culture with HS- 5 cells R 1400 2100 BCL2A1 BCL-xL T R 1000 1500 T Relative mRNA levels T 600 900 200 300 0 0 OCI-LY10 OCI-LY10 DOHH2 BJAB DOHH2 BJAB Supplementary Figure 3

  4. a 48h BJAB 48h b HS- 5 cells No Co-culture Co-culture Co-culture + YC137 (2.5M) 120 100 100 * 80 * * 80 % Viability * 60 % Viability ** 60 ** 40 * ** 40 20 20 0 Control YC137 (2.5µM) 0 Control 0.25 0.5 0.05 0.1 Doxorubicin (g/mL) Methotrexate (g/mL) Supplementary Figure 4

More Related