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Preferences of lab-raised and wild Armadillidium vulgare exposed to different colours of light. Kelsey Eatmon , Amie MacDonald and Elise Tessier BIOL 3401 November 2013. Outline. Background information Goal of our experiment Hypotheses Materials and Methods Results Discussion
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Preferences of lab-raised and wild Armadillidiumvulgareexposed to different colours of light Kelsey Eatmon, Amie MacDonald and Elise Tessier BIOL 3401 November 2013
Outline • Background information • Goal of our experiment • Hypotheses • Materials and Methods • Results • Discussion • Conclusions
Background Information • Pillbugs • Terrestrial isopods • Modified lungs • Sensitive to high intensities of light • Believed to be negatively-photosensitive http://en.wikipedia.org/wiki/Armadillidiidae
Goal • Determine if the photosensitivity of pillbugs raised in the wild and lab-raised pillbugs will increase as the wavelength of light decreases
Hypotheses • Lab-raised pillbugs and wild pillbugs will prefer the dark environments • Lab-raised pillbugswill be less photosensitive to a high intensity light than wild pillbugs • Photosensitivity will increase as the wavelength of light decreases
Materials and methods • Wild pillbugs were collected by Silver Lake in Sackville, New Brunswick • Maintained in a dark environment at an outdoor temperature
Materials and methods • Lab raised pillbugs came from the Animal Behaviour Lab at Mount Allison University • Maintained at room temperature • Regularly exposed to the light from the windows and the overhead lights (~340 Lux)
Materials and methods • Experimental Design • Elongated plexiglass chambers • One lamp placed on either end of the chamber • Filters used to produce the blue, yellow, and red light (43 Lux)
Materials and methods • 10 wild pillbugs and 10 lab-raised pillbugs tested separately • Monitored under sequential combinations of light colours
Specific colour preferences Visible light spectrum: DARK → RED → YELLOW → BLUE →WHITE Wild pillbugs: DARK → RED → BLUE → YELLOW → WHITE Lab-raised pillbugs: • Did not show pattern
Discussion • Acclimation • Social Behaviour • Aggregation patterns http://www.floridapest.com/pest_guide/landscape-pest/sow-bug.html
Discussion • Appearance of colours • Blue appears darker than yellow • Colours may not appear the same to pillbugs • Compound eye • Desiccation http://www.flickr.com/photos/54475261@N02/6736802539/
Conclusions • Wild pillbugs followed the predicted pattern • Exception: blue and yellow • Lab-raised pillbugs did not exhibit predicted behaviour • Acclimated to higher light intensities