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Magnetic Tweezer System for Single-DNA Experiments: A Summer Undergraduate Project

Magnetic Tweezer System for Single-DNA Experiments: A Summer Undergraduate Project. James Kath (REU), John Graham (Mentor) and John F. Marko Northwestern University Materials Research Science & Engineering Center Monica Olvera, Northwestern University, DMR 0520513.

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Magnetic Tweezer System for Single-DNA Experiments: A Summer Undergraduate Project

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  1. Magnetic Tweezer System for Single-DNA Experiments: A Summer Undergraduate Project James Kath (REU), John Graham (Mentor) and John F. Marko Northwestern University Materials Research Science & Engineering Center Monica Olvera, Northwestern University, DMR 0520513 REU student James Kath, under the supervision of Professor John Marko has constructed a magnetic tweezer system which allows force-extension and twisting experiments to be carried out on single DNA molecules. The primary application of this instrument will be to the study of protein-DNA interactions. Preliminary studies have verified that the new instrument can carry out rapid acquisition of extensions of DNA molecules, via particle-tracking and out-of-focus bead image analysis. This simple instrument can monitor motions of paramagnetic colloidal particles attached to DNA molecules in three dimensions. REU participant, James Kath constructs microscope which uses optical microscopy to monitor motions of magnetic particles attached to DNA molecules. Particles are pulled and rotated using permanent magnets on a positioner.

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