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Population Growth in Lemna minor. Pre-Lab: Important Notes & Homework. Safety: goggles, aprons, gloves Data collection: EVERY DAY for 1 st week HW:
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Pre-Lab: Important Notes & Homework • Safety: goggles, aprons, gloves • Data collection: EVERY DAY for 1st week • HW: • Become familiar with background info & procedures(S-1 – S-3 & S-5 – S-9). If anything is unclear, write it down for discussion on 10/28. • Copy Data tables, pre-lab questions (S-4) and post lab questions (S-10) into your notebook • Draw proper measuring technique for pipette, beaker, graduated cylinder
Pre-Lab: Class work • Lecture / discussion • Each person will complete the following tasks in their own notebook. Feel free to discuss with group mates. • Answer the Pre-lab questions found on S-4 (you should have already copied the questions.) • Draw pictures of the three sets of test tubes you are establishing. Make sure to label contents of each set. • Draw figure 3
Lecture / Discussion • Several Objectives • How L. minor population growth rate reacts in differing environments (pollution issues) • Determining r and K (biotic potential and its relationship to population growth ) • Practice with designing / running an experiment (practice for Paper 3)
Lecture / Discussion • Why need a control? • Why have replicates? • What is carrying capacity? • What part of the curve shows carrying capacity? • Is that in a logistic growth curve or exponential growth curve? • What is biotic potential?
Determining Population Size • Where, • d means change • N means Number of individuals • Nt is N at any given time (t) • N0 is N at time = zero • r is a constant: population’s intrinsic rate of increase • rm = biotic potential • K is carrying capacity • e is Euler’s constant (number) = 2.72
Exponential population growth equation: • dN/dt=rN • Nt = N0ert • Logistic population growth equation: • dN/dt = rN [(K-N)/K] Allows us to determine intrinsic rate. Or if given r, can predict N at future time.
Drawings • Test tubes • the water? • L. minor? • Any treatments:pH buffer, solutions, screens & rubberbands? • Plastic wrap with holes & rubberbands? • Beaker, graduated cylinder, pipette • The meniscus? • Pipette at 90° angle to tabletop?
Data Tables • Three different tests x three replicates = 9 test tubes • Need a data table / each test tube