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Seeding

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Seeding

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  1. hTERT, Survivin and VEGF as antisense targets in bladder cancer cells: antiproliferative and chemosensitizing effectsA. Meye, S. Füssel, K. Krämer, S. Krause, J. Herrmann, S.Ning, Y. Burmeister,M. Kotzsch#, B. Schwenzer*, O.W. Hakenberg, M.P. WirthKlinik für Urologie, Institut für Pathologie#, Institut für Biochemie*, TU Dresden Harnblasenkarzinom (BCa)  hohe Rezidiv- und Progressionsraten  häufig TherapieresistenzenAlternative: Ausschaltung von tumorrelevanten Genen mittelsAntisense-Desoxynukleotiden (AS-ODN)Ziel: tumorspezifischere Therapie & ChemosensitivierungKandidaten: hTERT (human telomerase reverse transcriptase) Survivin (inhibitor of apoptosis protein)  VEGF (vascular endothelial growth factor)

  2. 24h 24h 24h incubation for further CDDP, GEM 24, 48 or 72h 72h 20h Seeding CT Transfection WST WST - - 1 1 assay assay Cell counting Cell counting (4h) 2h 2h 2h Apoptosis detection Apoptosis detection MMC MMC Cell cycle analysis • AS-ODN gegen hTERT, Survivin & VEGF hemmen als Einzel-behandlung die Proliferation von versch. BCa-Zelllinien • Viabilität , Zellzahl , klonogenes Überleben , Apoptose, Zellzyklusarrest •  Chemosensitivierung durch AS-ODN-Vorbehandlung ?

  3. relative viability (%) Ja! signifikant stärkere CT-Wirkung nach Vorbehandlung mit AS-ODN gegen hTERT, Survivin & VEGF  geringere CT-Dosen nötig, weniger Nebeneffekte

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