1 / 13

Mouse Genome CGH Microarray 44A

Mouse Genome CGH Microarray 44A. Mouse Genome CGH Microarray Kit 44A Designed for CGH, Validated with samples of known aberrations. Only commercially available Mouse CGH Microarray !. Designed for genome-wide profiling of genomic aberrations on a single chip.

tamra
Download Presentation

Mouse Genome CGH Microarray 44A

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. Mouse Genome CGH Microarray 44A

  2. Mouse Genome CGH Microarray Kit 44ADesigned for CGH, Validated with samples of known aberrations Only commercially available Mouse CGH Microarray ! Designed for genome-wide profiling of genomic aberrations on a single chip. • Genome-wide coverage with average resolution ~35kb • Based on UCSC Genome Browser mm5 (NCBI Build 33) • All probes and annotations mapped to mm6 (NCBI Build 34) • Probe selection biased toward genes (73% intragenic vs. 27% intergenic) • Optimized probe design for aCGH application • Similar probe design and validation process as used in Human CGH array • Include CGH eQC grid • Compatible with current Agilent microarray platform and CGH protocols Part number: G4414A List price: $3750/Kit with volume break Discount: EDU (20%) and VEU Standard catalog array packaging

  3. Mouse Genome CGH Microarray Kit 44ADesigned for CGH, Validated with samples of known aberrations • Consisting of ~43,000 60-mer oligonucleotide probes on 1”x3” slide • Genome-wide coverage with average resolution ~35kb* • Content source: UCSC Genome Browser mm5 (NCBI Build 33) • All probes and annotations mapped to latest NCBI Build 34 • Probe selection biased toward genes 1 probe per well-characterized gene 2 or more probes for each of ~1100 cancer genes Intragenic probes (73%); intergenic probes (27%) • Optimized probe design for CGH application • Ability to detect single copy and homozygous deletions as well as low and high level amplifications • Compatible with current Agilent microarray platform and protocols * Calculated by using the total size of the non-repeated genome divided by the total # of features

  4. Probe Design Methodology • Tiled candidate probes across non-repeat masked regions across the genome in 30 bp steps between cut sites for restriction enzymes (RsaI and AluI) – sourced from UCSC Genome Browser mm5 (NCBI Build 33) • Performed homology search and retained highly specific probes • Filtered homology-searched probe set on the same in silico parameters as used in designing Human CGH array • Selected the best set of ~ 80,000 semifinal probes biasing toward genes & exons • Performed empirical validation using normal M/F cell-line samples and outliers were eliminated • Remove any probes that do not map to NCBI Build 34 • Final probe set within a narrow Tm range

  5. Genomic Coverage and Probe Distribution Total # of Features 44,290 # of Biological Probes 43,020 # of Unique Biological Probes 42,404 # of Replicated Biological Probes 308 x 3 Total # of Control probes 1,270 ___________________________________________ Intragenic probes 31,552 (73%) Exonic probes 17,344 (55%) Intronic probes 14,208 (45%) Intergenic probes 11,468 (27%)

  6. Mouse CGH Array Processing Oligo aCGH v2.0 protocol (Supplement with following material changes) Mouse Cot-1 DNA (1.0mg/ml) Invitrogen p/n 18440-016 C57BL/6J Genomic DNA: Male Jackson Labs p/n 000664 C57BL/6J Genomic DNA: Female Jackson Labs p/n 000664

  7. Mouse Genomic DNA Un-amplified Phi29 amplified Un-cut Un-cut Digested Digested

  8. Separation Histogram autosome probes X probes 3ug gDNA into Direct Labeling

  9. Ts65Dn Mouse • Ts65Dn mice are trisomic for the genes carried in the small translocation chromosome, symbolized Ts(1716)65Dn, that are normally located on Chr16. • Learn more from www.jax.org

  10. Ts(1716)65Dn Chromosome 16 • Polarity=1 Polarity=-1 Combined 3ug gDNA into Direct Labeling

  11. Ts(1716)65Dn Chromosome 16 Breakpoint • T65Dn translocation breaks mouse Chr 16 just proximal to the amyloid precursor protein (App) gene and contains the HSA21-homologous genes from App to the telomere.

  12. Aberrations in Lymphoma Cell Line 1(Combined dye-swaps; 10pt. Moving average) Collaboration Data from Lynda Chin, DFCI

  13. Aberrations in Lymphoma Cell Line 2(Combined dye-swaps; 10pt. Moving average) Collaboration Data from Lynda Chin, DFCI

More Related