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Liposome AutoMaker Automatic and Precise Liposome Production

Liposome AutoMaker Automatic and Precise Liposome Production. Hashimoto Models. LipidFilmAutoMaker LiposomeAutoMaker AutoExtruder. Manual Methods. Imprecise Liposome Production. Reverse-phase evaporation method. Vortex-mixing method. LUV. MLV. Lipid/ chloroform.

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Liposome AutoMaker Automatic and Precise Liposome Production

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  1. Liposome AutoMakerAutomatic and Precise Liposome Production

  2. Hashimoto Models LipidFilmAutoMaker LiposomeAutoMaker AutoExtruder

  3. Manual Methods Imprecise Liposome Production Reverse-phase evaporation method Vortex-mixing method LUV MLV Lipid/ chloroform Diameter : 100-1,000 nm Gentle hydration method GUV Sonication method SUV Diameter : 20-100 nm Diameter : 5-100 μm Rotary evaporator

  4. Manual Methods MLV Lipid/ chloroform SUV Vortex Mixer Lipid/ Ether/ Buffer ＋Ether LUV Rotary Evaporator Sonicator Rotary Evaporator GUV Buffer Natural Swelling

  5. Manual Methods Problems • Limited Functional Liposome Capabilities • Production Inefficiency • Encapsulating Inefficiency • Delivery Inefficiency • Lack of Repeatability

  6. Solution - Automated Method Multi-Functional Liposome AutoMaker

  7. AutoMaker Liposomes Examples Reconstituted Liposome Liposome Vaccine GUV SUV LUV MLV

  8. AutoMaker Advantages Multiple Functional Liposome Capabilities • Type: MLV, SUV, LUV, GUV • Sensitive to Temperature, pH, Magnetism, Ultrasound • Coatings: PEG, Saccharide Chains • Bound Ligands: Proteins, Peptides, Nucleic Acids • Reconstituted Liposomes: Proteoliposomes and Virosomes • Liposome Vaccines

  9. AutoMaker Advantages Production Efficiency • Semi-Skilled Users Can Reproduce Every Variety of Liposomes • Automated Operation under Atmospheric Nitrogen • Automated Cleaning and Drying Using Purified Water and Ethanol • 20-40 ml per Operation • 10 Runs per Day

  10. Encapsulating Efficiency AutoMaker Advantages Liposomes encapsulate a variety of molecules with high trapping efficiency.

  11. AutoMaker Advantages Delivery Efficiency • Liposome Characteristics Can Vary with Target • Sensitive to Temperature, pH, Magnetism, Ultrasound • Delivery Coatings: PEG, Saccharide Chains • Bound Ligands: Proteins, Peptides, Nucleic Acids

  12. AutoMaker Advantages Repeatability • Liposome Production Details Can Be Recorded • Reproducibility Is Measured • Liposome Characteristics Can Be Demonstrated

  13. Baculovirus gene expression system Membrane protein gene Recombinant membrane protein-presenting budded virus ＋ Fusion Liposome 10μm Recombinant proteoliposome Application Example Recombinant Proteoliposomes

  14. Microtiter plate Recombinant membrane receptor-presenting liposome-fixed ELISA plate Application Example Recombinant Membrane Receptor-Presenting Liposomes ELISA Fixation Substate Product Substrate Product Y Y Enzyme-linked secondary antibody Enzyme-linked secondary antibody Y Y Autoantibody Anti-ligand antibody Specific ligand TSHR：Graves’ disease AChR：Myasthenia gravis Membrane receptor Recombinant membrane receptor- presenting liposomes Recombinant membrane receptor- presenting liposomes Biotin Biotin Streptavidin Streptavidin

  15. ELISA Data for Patients with Thyroid Diseases Proteoliposomal ELISA RadioImmunoAssay Graves’ disease Hashimoto’s disease Normal

  16. Inactivated Bacteria Inactivated Virus Buffer Sonication MLV Lipid Film Lipid/Chloroform Vortex Mixing Bacterial surface antigen-presenting liposomes Viral surface antigen-presenting liposomes Application Example Ultrasonicated Liposomes

  17. Protein Binding ratio(%) Fab94 CRP 82 Application Example Protein-Bound Liposomes 2 + 2 2

  18. Application Example Sugar-Doped Lipid Film-Applied Liposomes Solvent removal Hydration Seepage stimulation Lipid＋Hexose in CHCl3/MeOH Hexose-doped lipid film DOPC/DOPG/Fru(10:1:10) Solubility in methanol Fructose ：～300 mM Mannose ：～100 mM Glucose ：～100 mM Galactose：～10 mM 50μm DOPC/Glc(1:1) DOPC 50μm

  19. Application Example Liposome Vaccines Bacteria Virus Iridovirus（Sea-bream, Yellowtail, etc.） Edwardsiella tarda（Flounder, Sea-bream, etc.） Aeromonashydrophila（Carp, Eel, etc.） Koi herpesvirus, KHV（Koi） Flavobacterium psychrophilum（Ayu, Rainbow trout, etc.） Influenza virus（Mankind）

  20. Hashimoto Models LipidFilmAutoMaker LiposomeAutoMaker AutoExtruder

  21. Liposome AutoMakerAutomatic and Precise Liposome Production

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