1 / 15

淫羊藿苷体外抑制 SIV 感染诱导的 CEMx174 细胞凋亡

淫羊藿苷体外抑制 SIV 感染诱导的 CEMx174 细胞凋亡. Ic-ariin Inhibits the Apoptosis of CEM x174 Triggered by Simian Immunodeficiency Virus Infection in vitro. Immunopathogenicity of HIV infection. Classical Apoptotic Pathway. Another pathway: cAMP-PKC pathway. Activities of Epimedium brevicormon Maxim.

tanner
Download Presentation

淫羊藿苷体外抑制 SIV 感染诱导的 CEMx174 细胞凋亡

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. 淫羊藿苷体外抑制SIV感染诱导的CEMx174细胞凋亡淫羊藿苷体外抑制SIV感染诱导的CEMx174细胞凋亡 Ic-ariin Inhibits the Apoptosis of CEM x174 Triggered by Simian Immunodeficiency Virus Infection in vitro

  2. Immunopathogenicity of HIV infection

  3. Classical Apoptotic Pathway

  4. Another pathway: cAMP-PKC pathway

  5. Activities of Epimedium brevicormon Maxim • 补肾壮阳、祛风除湿 • Enhance immune function • Activate sexual gland • Anti-aging • Antiviral activity?

  6. Drug toxicity to CEM x174 • Identification of SIV infection • Inhibitory effect of Ic-ariin on replication of SIV • The apoptosis analysis was performed by Annexin V and Propidiumiodide (PI) double dying. • cAMP:radioimmunoaasay • PKA activity:Western blotting

  7. 接种SIV病毒一周后的细胞病理变化syncytia formation (CPE)

  8. Amplification of the SIVmac251 gag gene segment.

  9. Inhibitory effect of Ic-ariin on replication of SIV • 其半数毒性浓度(Toxicity concentration, TC50)为134ug/ml • 半数抑制浓度(Inhibitory concentration,IC50)为26ug/ml， • 治疗指数（Theraputic index,TI）为5.15 • 在亚急性浓度下，淫羊藿甙对SIV复制具有良好的剂量依赖关系 。

  10. group LL LR UR UL Effect of Ic-ariin on apoptosis of normal and SIVmac251 infectedCEM x174 cells nornal cell no85.56±0.686.69±0.386.44±0.111.31±0.10 Ic-arrin 89.45±1.03*4.21±0.19 **5.16±0.11**1.19±0.04 Infectious cell no77.71±0.7510.77±1.1210.25±0.321.28±0.06 Ic-arrin 86.11±1.20**6.97±0.29 **5.96±0.23**0.97±0.15 LL live cell； LR apoptosis cell ；UR death cell ；UL damage cell

  11. Effects of Ic-ariin on the concentration of cAMP in normaland SIVmac251 1nfected CEM x174 cells

  12. Effects ofIc-ariin on PKA activity in normal and SIVmac251 1nfected CEM x174 cells

  13. Conclusion • Ic-ariin inhibits the replication of SIV dose-dependently • Ic-ariin may transiently delay the apoptosis of CEM x174 triggered by SIVmac251. • Ic-ariin increased the percentage of viable cells while also decreasing the percentage of apoptotic cells in both the normal and SIVmac251 infected groups. But the effect of Ic-ariin in the SIVmac251 infected group was more marked than in normal cells. • Activating the cAMP-PKA signaling pathway might be one of the inhibitory mechanism for the apoptosis of CEM x174 triggered by SIVmac251

  14. 广州中医药大学热带医学研究所Tropical medicine InstituteGuangzhou Univ.of Chinese Medicine • 张奉学符林春 • 邓文娣吴爱武王新华 • 卢耀增、吴小娴

  15. 谢谢 THANK YOU

More Related