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A. CMV. pCOR-kTg10. 3 ’ UTR. CMV. pCOR-hTg10. 3 ’ UTR. CMV-tTA. CMV. 3 ’ UTR. CMV-rtTA. CMV. 3 ’ UTR. mCMV. tetO. tetO-Tg10. 3 ’ UTR. Tg10 h. IRES. B. 1600. Tg10. 1. 27. k. 1400. 2. 28. 3. 29. 1200. 4. 30. Tg10 (ng/ml). 1000. 5. 31. Tg10. rtTA. tTA. h. 800.
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A CMV pCOR-kTg10 3 ’ UTR CMV pCOR-hTg10 3 ’ UTR CMV-tTA CMV 3 ’ UTR CMV-rtTA CMV 3 ’ UTR mCMV tetO tetO-Tg10 3 ’ UTR Tg10 h IRES B 1600 Tg10 1 27 k 1400 2 28 3 29 1200 4 30 Tg10 (ng/ml) 1000 5 31 Tg10 rtTA tTA h 800 600 400 200 0 0 20 40 60 80 100 120 140 160 Days after electroporation C 120 100 80 Tg10 (ng/ml) 60 40 Tg10 k 20 0 0 14 28 42 56 70 84 98 112 126 140 154 - dox + dox - dox + dox - dox Days after electroporation