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This gel depicts the NEB 1kb ladder and a digestion of the plasmid pIS1-Eef25UTR-renilla with the following enzymes: NcoI , NheI , Ncol-HF+Nhel-HF. Plasmid 38235 pIS1-Eef25UTR-renilla. 1kb Ladder. Ncol -HF. Nhel -HF. Ncol+Nhel HF. Uncut.
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This gel depicts the NEB 1kb ladder and a digestion of the plasmid pIS1-Eef25UTR-renillawith the following enzymes: NcoI, NheI, Ncol-HF+Nhel-HF Plasmid 38235 pIS1-Eef25UTR-renilla 1kb Ladder Ncol-HF Nhel-HF Ncol+Nhel HF Uncut 0.5 1 1.5 2 3 4 5 6 8 10 DNA digested withNcoI, NheI, Ncol-HF+Nhel-HF for 2 hours Gel: 0.8% TAE stained with 100 ml 1X TAE and 5uL of ethidium bromide Ran for 2 hours at 60 volts