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A Map of the Interactome Network of the Metazoan C. elegans

A Map of the Interactome Network of the Metazoan C. elegans. Science , Vol 303, 540-543, 23 January 2004. how protein-protein interaction (or "interactome") networks relate to multicellular functions. The yeast Saccharomyces cerevisiae : develop a eukaryotic unicellular interactome map.

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A Map of the Interactome Network of the Metazoan C. elegans

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  1. A Map of the Interactome Network of the Metazoan C. elegans Science, Vol 303, 540-543, 23 January 2004

  2. how protein-protein interaction (or "interactome") networks relate to multicellular functions

  3. The yeast Saccharomyces cerevisiae : develop a eukaryotic unicellular interactome map. • Caenorhabditis elegans : studying how protein networks relate to multicellularity. • Investigate its interactome network with HT-Y2H.

  4. Y2H system • bioinformatics criteria • coaffinity purification (co-AP) glutathione S-transferase (GST) pull-down assay

  5. more than 4000 interactions were identified from high-throughput, yeast two-hybrid (HT=Y2H) screens • the Worm Interactome (WI5) map contains 5500 interactions

  6. Coaffinity purification assays

  7. Decision tree used to assign a confidence level to Y2H interactions.

  8. Analysis of the WI5 network

  9. Overlap with transcriptome The dash-circled proteins belong to the same paralogous family (sharing more than 80% homology)

  10. In S. cerevisiae, two proteins that have many interaction partners in common are more likely to be related biologically

  11. These data demonstrate that their three data sets contain a large proportion of highly reliable interactions and corroborate their expected relative qualities. • Topological and biological features of this interactome network, as well as its integration with phenome and transcriptome data sets, lead to numerous biological hypotheses.

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