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Appendix. 2D SDS-PAGE protein profiles of 35 individual bulls.

Appendix. 2D SDS-PAGE protein profiles of 35 individual bulls. 10. 3. pH. 10. kDa. kDa. 3. pH. 200. 200. 99. 99. 66.2. 66.2. 45. 45. 31. 31. 21. 21. 14. 14. 6.5. 6.5. FH3483. FH2799. 200. 200. 99. 99. 66.2. 66.2. 45. 45. 31. 31. 21. 21. 14. 14. 6.5. 6.5.

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Appendix. 2D SDS-PAGE protein profiles of 35 individual bulls.

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  1. Appendix. 2D SDS-PAGE protein profiles of 35 individual bulls. 10 3 pH 10 kDa kDa 3 pH 200 200 99 99 66.2 66.2 45 45 31 31 21 21 14 14 6.5 6.5 FH3483 FH2799 200 200 99 99 66.2 66.2 45 45 31 31 21 21 14 14 6.5 6.5 FH2134 FH3579 200 200 99 99 66.2 66.2 45 45 31 31 21 21 14 14 6.5 6.5 FH3484 FH2208 • Appendix

  2. Appendix. 2D SDS-PAGE protein profiles of 35 individual bulls. 10 10 3 pH 3 kDa pH kDa 200 200 99 99 66.2 66.2 45 45 31 31 21 21 14 14 6.5 6.5 FH2196 FH3490 200 200 99 99 66.2 66.2 45 45 31 31 21 21 14 14 6.5 6.5 FH2196 FH3579 200 200 99 99 66.2 66.2 45 45 31 31 21 21 14 14 6.5 6.5 FH3490 FH3485 • Appendix

  3. Appendix. 2D SDS-PAGE protein profiles of 35 individual bulls. 10 10 3 3 pH pH kDa kDa 200 200 99 99 66.2 66.2 45 45 31 31 21 21 14 14 6.5 6.5 FH3485 FH3523 200 200 99 99 66.2 66.2 45 45 31 31 21 21 14 14 6.5 6.5 FH3523 FH3490 200 200 99 99 66.2 66.2 45 45 31 31 21 21 14 14 6.5 6.5 FH3486 FH3507 • Appendix

  4. Appendix. 2D SDS-PAGE protein profiles of 35 individual bulls. 10 10 3 3 pH pH kDa kDa 200 200 99 99 66.2 66.2 45 45 31 31 21 21 14 14 6.5 6.5 FH3500 FH3485 200 200 99 99 66.2 66.2 45 45 31 31 21 21 14 14 6.5 6.5 FH3492 FH3485 200 200 99 99 66.2 66.2 45 45 31 31 21 21 14 14 6.5 6.5 FH3490 FH2799 • Appendix

  5. Appendix. 2D SDS-PAGE protein profiles of 35 individual bulls. 10 10 3 3 pH pH kDa kDa 200 200 99 99 66.2 66.2 45 45 31 31 21 21 14 14 6.5 6.5 FH2208 FH2732 200 200 99 99 66.2 66.2 45 45 31 31 21 21 14 14 6.5 6.5 BB1022 FH2134 200 200 99 99 66.2 66.2 45 45 31 31 21 21 14 14 6.5 6.5 AA0881 FH2799 • Appendix

  6. Appendix. 2D SDS-PAGE protein profiles of 35 individual bulls. 10 10 3 3 pH pH kDa kDa 200 200 99 99 66.2 66.2 45 45 31 31 21 21 14 14 6.5 6.5 FH3484 BB1024 200 200 99 99 66.2 66.2 45 45 31 31 21 21 14 14 6.5 6.5 BB0374 FH3550 200 99 66.2 45 31 21 14 Appendix 4. Separation by 2D SDS PAGE. Protein (300µg) was added to IPG strips, separated by isoelectric focusing then loaded onto a 15% (w/v) large format acrylamide gel. Gels were run at 30mA for 30min followed by 60mA for 4h. 6.5 FH2451 • Appendix

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