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Pan Pacific Pork Expo 2006. Pig Reproduction. M.E. Wilson. 30 Pigs weaned/ sow/ year. PMWS. Economics of reproduction. 3-3.5 US cents per market pig/EBV point above the average $2.35 improvement in each generation. Natural Mating. Natural Service vs Artificial Insemination.
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Pan Pacific Pork Expo 2006 Pig Reproduction M.E. Wilson
Economics of reproduction • 3-3.5 US cents per market pig/EBV point • above the average • $2.35 improvement in each generation
Natural Mating Natural Service vs Artificial Insemination
Which Boar will have the Highest Fertility? Lebowa and Wilson, unpublished data, 2005
Mean Fertility Results Lebowa and Wilson, unpublished data, 2005
Effects of heterospermic matings verses homospermic matings on litter size Number of Matings Litter Size % conception Single Boar 93 79.6 9.26 Two different Boars 106 89.6 10.68 Thacker and Kirkwood, 1988
Comparison of Breeding Methods Number of Farrowing Total born Method Litters rate % - mummy AI- 2 different A 220 88 12.36 boars Combo - 1 A 218 88 12.06 B natural 1AI A Natural serv 189 88 11.9 B AI- 1boar 185 88 11.62 B Different superscripts P<.05 United Feeds, 1998
UltrasonographyMonitoring Ovarian Activity No Follicles Kemp and Soede, 1996 Follicles follicles
The effects of the interval between insemination and ovulation on Fertilization rate and embryo viability (Soede, et.al., 1995a)
Synchronized Estrus and ovulation • 500 – 750 IU of eCG Pregnecol (Canada) • 400 IU eCG and 200 IU hCG (PG 600) • 42 hours after hCG ovulation typically occurs • 36-38 hours after pLH ovulation will be induced
The issue is not TECHNOLOGY The issue is IMPLEMENTATION
Artificial Insemination Transcervical Traditional Deep Uterine
The number of pigs per 100 matings with 0.5, 1 or 4 billion cells transcervical1 or 4 billion cells intra cervical 1 Transcervical catheter passage was 94%. Hemorrhaging observed on Catheter 4% of matings (n=620) K. Rozeboom
Sexed Sperm Cells • Non-surgical implantation of embryos • Frozen Semen • Expanding genetic potential and reducing • variation
Sperm sexing technology • Based on the fact that more (2.8% – 7%) DNA is contained in X-bearing than in Y-bearing sperm
Litter of 14 pigs from non-surgical transfer 15 embryos transferred 14 pigs born alive
Dolly, the sheep (1996) Cloning First mammal to be cloned from adult cells (mammary) Surrogate mother Dolly
Cloned embryos Transferred within 24 hrs - Porcine Surgical transfer into oviduct- 50-100 clones Chromatin Transfer Viable cloned offspring (litter size ave. = 4-8) Adult donor
Benefits of Cloning • Advance superior genetics • Feed efficiency • Rate of gain • Carcass traits • Gene markers for disease • Research model • Removes more variation • Medical models • Testing human diseases