1 / 21

PTT 203 Biochemical Engineering

PTT 203 Biochemical Engineering. Kinetics MICROBIAL GROWTH IN continuous CULTURE. WEEK 11-12 By : MADAM NOORULNAJWA DIYANA YAACOB. Lecture Outline. Introduction Continuous Growth Characteristics Dilution Rate, Optimum Operation. Cell growth in Continuous Culture. Cell growth.

tavita
Download Presentation

PTT 203 Biochemical Engineering

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. PTT 203Biochemical Engineering Kinetics MICROBIAL GROWTH IN continuous CULTURE WEEK 11-12 By: MADAM NOORULNAJWA DIYANA YAACOB

  2. Lecture Outline • Introduction • Continuous Growth Characteristics • Dilution Rate, Optimum Operation

  3. Cell growth in Continuous Culture

  4. Cell growth • The net specific growth rate is the difference between a gross specific growth rate (μg, h-1) and the rate of loss of cell mass due to cell death (kd, h-1): • Microbial growth can also be described in terms of cell number, N: where μR is the net specific replication rate (h-1)

  5. Chemostat as Tool

  6. Chemostat Mass Balance

  7. Ideal Constant- Stirred tank Reactor Chemostat

  8. MASS BALANCE STATEMENT FOR CELL MASS

  9. Steady State and Sterile Feed

  10. Substrate Concentration

  11. Bioreactor Washout Condition

  12. With Endogenous Metbolism

  13. Cell Concentration

  14. Cell concentration (cont.)

  15. Effect of Endogenous Metabolism

  16. Measurement of Maximum Cell Yield and Maintenance a Chemostat

  17. Using a Chemostat to Determine µmax and KS

  18. Productivity of a Chemostat

  19. Product Mass Balance

  20. Product Mass Balance

  21. A summary of Chemostat Response

More Related