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Extracting Chlorophyll Using paper chromatography

Extracting Chlorophyll Using paper chromatography. The chemical formula for water =. H 2 O. Hydrogen = H. H 2 = 2 atoms of Hydrogen. Oxygen = O. H 2 O. Extracting Chlorophyll Using paper chromatography. The chemical formula for carbon dioxide =. CO 2. Carbon = C. Oxygen = O.

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Extracting Chlorophyll Using paper chromatography

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  1. Extracting ChlorophyllUsing paper chromatography The chemical formula for water = H2O Hydrogen = H H2 = 2 atoms of Hydrogen Oxygen = O H2O

  2. Extracting ChlorophyllUsing paper chromatography The chemical formula for carbon dioxide = CO2 Carbon = C Oxygen = O O2 = 2 atoms of Oxygen CO2

  3. Extracting ChlorophyllUsing paper chromatography Scientists write equations to show chemical reactions The formation of water from hydrogen and oxygen is written as: 2H2 + O2 2H2O + energy

  4. Extracting ChlorophyllUsing paper chromatography Scientists write equations to show chemical reactions 2H2 + O2 2H2O + energy Two molecules of water are formed Energy is released

  5. Extracting ChlorophyllUsing paper chromatography The most important chemical reaction in the world is… PHOTOSYNTHESIS The chemical reaction for photosynthesis is 6CO2 + 6H2O + energy C6H12O6 + 6O2

  6. Extracting ChlorophyllUsing paper chromatography PHOTOSYNTHESIS Sugar Carbon Dioxide 6CO2 + 6H2O + energy C6H12O6 + 6O2 Oxygen Sunlight Water

  7. Extracting ChlorophyllUsing paper chromatography PHOTOSYNTHESIS Plants possess a light trapping molecule called Chlorophyll

  8. Extracting ChlorophyllUsing paper chromatography PHOTOSYNTHESIS Chlorophyll Captures light energy and uses it to make food Gives leaves their green color

  9. Extracting ChlorophyllUsing paper chromatography PHOTOSYNTHESIS Chlorophyll Gives leaves their green color Comes in many colors and two forms Chlorophyll a Chlorophyll b

  10. Extracting ChlorophyllUsing paper chromatography PHOTOSYNTHESIS Chlorophyll Chlorophyll a Chlorophyll b Both forms absorb different wavelengths of light

  11. Extracting ChlorophyllUsing paper chromatography PHOTOSYNTHESIS Chlorophyll In this paper chromatography experiment Will appear as a bright yellow-green band Chlorophyll a Will appear as a dull green band Chlorophyll b

  12. Extracting ChlorophyllUsing paper chromatography Accessory Pigments that help transfer light to the chlorophyll Will appear as an orange band Carotene Will appear as a yellow band Xanthophyll Will appear as a red-brown band Anthocyanin (acts as plant sunscreen)

  13. Extracting ChlorophyllUsing paper chromatography Materials Filter paper cut into strips Pennies Straw or stirrer Beaker Rubbing alcohol Ruler Tape Plant leaves

  14. Extracting ChlorophyllUsing paper chromatography Procedure Draw a line across the paper strip, 2 cm from one end Place the leaf you want to test on top of the filter paper strip, remembering where the line is

  15. Extracting ChlorophyllUsing paper chromatography Procedure Using the edge of a penny, trace the penny across the leaf right on top of where you drew the line Push down hard enough to leave a green smear on the paper on top of the pencil mark

  16. Extracting ChlorophyllUsing paper chromatography Procedure Repeat 2 more times with fresh sections of leaf to make the smear darker Try to keep the smear as close to the original pencil line as possible

  17. Extracting ChlorophyllUsing paper chromatography Procedure Fill the beaker to a depth of 1 cm with rubbing alcohol Carefully lower the filter paper strip, green smear end in first, into the beaker until the bottom just touches the alcohol

  18. Extracting ChlorophyllUsing paper chromatography Procedure Make sure that the green smear does not actually touch the alcohol, only the tip of the paper Lay the stick across the mouth of the beaker like a bridge from one edge to the other

  19. Extracting ChlorophyllUsing paper chromatography Procedure Tape the filter paper strip to the stick so that the paper is held in place just touching the alcohol but not touching the sides of the beaker

  20. Extracting ChlorophyllUsing paper chromatography

  21. Extracting ChlorophyllUsing paper chromatography Procedure Wait. The alcohol should gradually move up the paper, bringing many of the pigments along with it Remove the stick and paper when the alcohol has almost reached the stick

  22. Extracting ChlorophyllUsing paper chromatography Procedure Mark how far up the paper the alcohol traveled with a pencil Determine how many bands of pigment you have, what color they are, and measure how far each band traveled from the origin line

  23. Extracting ChlorophyllUsing paper chromatography Procedure Identify the pigments you found based on their colors Draw a color chart of what you found Prepare a report out for the class

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